Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population

Khalid M. Alkharfy, Nasser M. Al-Daghri, Omar S. Al-Attas, Majed S. Alokail, Abdul Khader Mohammed, Benjamin Vinodson, Mario Clerici, Usamah Kazmi, Tajamul Hussain, Hossam M. Draz

Research output: Contribution to journalArticle

Abstract

Genetics plays a crucial role in the development of metabolic syndrome (MetS). Here we examined the association between endothelial nitric oxide synthase (eNOS) gene polymorphisms and MetS in a Saudi Arabian cohort to extend the understanding of the genetic basis of MetS in diverse ethnic populations. Anthropometric, clinical and biochemical parameters as well as genotyping for 894G>T, -786T>C variants of eNOS gene by PCR-RFLP and 4a/b by direct PCR were performed in 886 Saudi Arabians (477 MetS and 409 Non-MetS). The genotype distribution (TT, p=0.001; TC, p=0.001; TC+CC, p=0.001) and allele (T, p=0.007; C, p=0.007) frequency of the -786T>C SNP were significantly different between Non-MetS and MetS subjects which remained significant after Bonferroni correction. Moreover: 1) the GT and GT+TT genotypes of the 894G>T SNP were associated with elevated blood pressure (p=0.017, and p=0.022, respectively); 2) the ab variant of 4a/b polymorphism was associated with decreased HDL levels (p= 0.044); and 3) the TC+CC genotype and C allele of the -786T>C SNP were associated with increased fasting glucose levels (p=0.039, and p=0.028, respectively). Also, G-a-C was identified as the risk haplotype for MetS susceptibility (p=0.034). The results suggest a significant association of 894G>T, 4a/b and -786T>C polymorphisms with MetS and its components is present in an Arab population. A genetic predisposition to develop abnormal metabolic phenotypes, consistent with an increased prevalence of metabolic phenotypes can be detected in this ethnic group.

Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalEndocrine Journal
Volume59
Issue number3
DOIs
Publication statusPublished - 2012

Fingerprint

Nitric Oxide Synthase Type III
Population
Genes
Single Nucleotide Polymorphism
Genotype
Alleles
Phenotype
Polymerase Chain Reaction
Genetic Predisposition to Disease
Ethnic Groups
Restriction Fragment Length Polymorphisms
Haplotypes
Fasting
Blood Pressure
Glucose

Keywords

  • Gene polymorphism
  • Metabolic syndrome
  • Nitric oxide synthase
  • Saudi Arabians

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Alkharfy, K. M., Al-Daghri, N. M., Al-Attas, O. S., Alokail, M. S., Mohammed, A. K., Vinodson, B., ... Draz, H. M. (2012). Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population. Endocrine Journal, 59(3), 253-263. https://doi.org/10.1507/endocrj.EJ11-0278

Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population. / Alkharfy, Khalid M.; Al-Daghri, Nasser M.; Al-Attas, Omar S.; Alokail, Majed S.; Mohammed, Abdul Khader; Vinodson, Benjamin; Clerici, Mario; Kazmi, Usamah; Hussain, Tajamul; Draz, Hossam M.

In: Endocrine Journal, Vol. 59, No. 3, 2012, p. 253-263.

Research output: Contribution to journalArticle

Alkharfy, KM, Al-Daghri, NM, Al-Attas, OS, Alokail, MS, Mohammed, AK, Vinodson, B, Clerici, M, Kazmi, U, Hussain, T & Draz, HM 2012, 'Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population', Endocrine Journal, vol. 59, no. 3, pp. 253-263. https://doi.org/10.1507/endocrj.EJ11-0278
Alkharfy, Khalid M. ; Al-Daghri, Nasser M. ; Al-Attas, Omar S. ; Alokail, Majed S. ; Mohammed, Abdul Khader ; Vinodson, Benjamin ; Clerici, Mario ; Kazmi, Usamah ; Hussain, Tajamul ; Draz, Hossam M. / Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population. In: Endocrine Journal. 2012 ; Vol. 59, No. 3. pp. 253-263.
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