Varicella zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique

G. Gerna, R. W. Chambers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new plaque assay for the quantitation of varicella zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. In six patients with zoster infection, neutralizing antibody titers ranged from 1:20 to 1:40 before the onset of infection and reached high levels (1:320 to 1:5,120) during the convalescent phase of the disease. Complement fixing (CF) titers were all negative (

Original languageEnglish
Pages (from-to)437-442
Number of pages6
JournalJournal of Clinical Microbiology
Volume4
Issue number5
Publication statusPublished - 1976

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Neutralization Tests
Chickenpox
Herpes Zoster
Neutralizing Antibodies
Immunoenzyme Techniques
Human Herpesvirus 3
Infection
Cell Culture Techniques

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Varicella zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique. / Gerna, G.; Chambers, R. W.

In: Journal of Clinical Microbiology, Vol. 4, No. 5, 1976, p. 437-442.

Research output: Contribution to journalArticle

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