Abstract
In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added to cultured endothelial cells, BV13 induces a redistribution of VE-cadherin from intercellular junctions. VE-cadherin redistribution did not change the localization of platelet endothelial cell adhesion molecule or tight junction markers such as zonula occludens 1, cingulin, and junctional adhesion molecule. Intravenous administration of mAb BV13 induced a concentration- and time-dependent increase in vascular permeability in heart and lungs. By electron microscopy, interstitial edema and accumulation of mixed types of inflammatory cells in heart and lungs were observed. Injection of (rhodamine-labeled) Ricinus communis I lectin showed focal spots of exposed basement membrane in the alveolar capillaries and in some larger pulmonary vessels. These data indicate that VE-cadherin is required for vascular integrity and normal organ functions.
| Original language | English |
|---|---|
| Pages (from-to) | 9815-9820 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 96 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Aug 17 1999 |
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Keywords
- Adherens junction
- Endothelium
- Permeability
ASJC Scopus subject areas
- Genetics
- General
Cite this
Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo. / Corada, Monica; Mariotti, Massimo; Thurston, Gavin; Smith, Kelly; Kunkel, Robin; Brockhaus, Manfred; Lampugnani, Maria Grazia; Martin-Padura, Ines; Stoppacciaro, Antonella; Ruco, Luigi; Mcdonald, Donald M.; Ward, Peter A.; Dejana, Elisabetta.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 17, 17.08.1999, p. 9815-9820.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo
AU - Corada, Monica
AU - Mariotti, Massimo
AU - Thurston, Gavin
AU - Smith, Kelly
AU - Kunkel, Robin
AU - Brockhaus, Manfred
AU - Lampugnani, Maria Grazia
AU - Martin-Padura, Ines
AU - Stoppacciaro, Antonella
AU - Ruco, Luigi
AU - Mcdonald, Donald M.
AU - Ward, Peter A.
AU - Dejana, Elisabetta
PY - 1999/8/17
Y1 - 1999/8/17
N2 - In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added to cultured endothelial cells, BV13 induces a redistribution of VE-cadherin from intercellular junctions. VE-cadherin redistribution did not change the localization of platelet endothelial cell adhesion molecule or tight junction markers such as zonula occludens 1, cingulin, and junctional adhesion molecule. Intravenous administration of mAb BV13 induced a concentration- and time-dependent increase in vascular permeability in heart and lungs. By electron microscopy, interstitial edema and accumulation of mixed types of inflammatory cells in heart and lungs were observed. Injection of (rhodamine-labeled) Ricinus communis I lectin showed focal spots of exposed basement membrane in the alveolar capillaries and in some larger pulmonary vessels. These data indicate that VE-cadherin is required for vascular integrity and normal organ functions.
AB - In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added to cultured endothelial cells, BV13 induces a redistribution of VE-cadherin from intercellular junctions. VE-cadherin redistribution did not change the localization of platelet endothelial cell adhesion molecule or tight junction markers such as zonula occludens 1, cingulin, and junctional adhesion molecule. Intravenous administration of mAb BV13 induced a concentration- and time-dependent increase in vascular permeability in heart and lungs. By electron microscopy, interstitial edema and accumulation of mixed types of inflammatory cells in heart and lungs were observed. Injection of (rhodamine-labeled) Ricinus communis I lectin showed focal spots of exposed basement membrane in the alveolar capillaries and in some larger pulmonary vessels. These data indicate that VE-cadherin is required for vascular integrity and normal organ functions.
KW - Adherens junction
KW - Endothelium
KW - Permeability
UR - http://www.scopus.com/inward/record.url?scp=13044264471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13044264471&partnerID=8YFLogxK
U2 - 10.1073/pnas.96.17.9815
DO - 10.1073/pnas.96.17.9815
M3 - Article
C2 - 10449777
AN - SCOPUS:13044264471
VL - 96
SP - 9815
EP - 9820
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 17
ER -