Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo

Monica Corada, Massimo Mariotti, Gavin Thurston, Kelly Smith, Robin Kunkel, Manfred Brockhaus, Maria Grazia Lampugnani, Ines Martin-Padura, Antonella Stoppacciaro, Luigi Ruco, Donald M. Mcdonald, Peter A. Ward, Elisabetta Dejana

Research output: Contribution to journalArticle

Abstract

In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added to cultured endothelial cells, BV13 induces a redistribution of VE-cadherin from intercellular junctions. VE-cadherin redistribution did not change the localization of platelet endothelial cell adhesion molecule or tight junction markers such as zonula occludens 1, cingulin, and junctional adhesion molecule. Intravenous administration of mAb BV13 induced a concentration- and time-dependent increase in vascular permeability in heart and lungs. By electron microscopy, interstitial edema and accumulation of mixed types of inflammatory cells in heart and lungs were observed. Injection of (rhodamine-labeled) Ricinus communis I lectin showed focal spots of exposed basement membrane in the alveolar capillaries and in some larger pulmonary vessels. These data indicate that VE-cadherin is required for vascular integrity and normal organ functions.

Original languageEnglish
Pages (from-to)9815-9820
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number17
DOIs
Publication statusPublished - Aug 17 1999

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Keywords

  • Adherens junction
  • Endothelium
  • Permeability

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Corada, M., Mariotti, M., Thurston, G., Smith, K., Kunkel, R., Brockhaus, M., Lampugnani, M. G., Martin-Padura, I., Stoppacciaro, A., Ruco, L., Mcdonald, D. M., Ward, P. A., & Dejana, E. (1999). Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo. Proceedings of the National Academy of Sciences of the United States of America, 96(17), 9815-9820. https://doi.org/10.1073/pnas.96.17.9815