Vascular endothelial growth factor A (VEGFA) expression in mycosis fungoides

Alessandro Pileri, Claudio Agostinelli, Simona Righi, Fabio Fuligni, Francesco Bacci, Elena Sabattini, Annalisa Patrizi, Stefano A. Pileri, Pier Paolo Piccaluga

Research output: Contribution to journalArticle

Abstract

Aims: High levels of vascular endothelial growth factor A (VEGFA) seem to herald a worse prognosis in mycosis fungoides (MF). In this study, we aimed to characterize more clearly VEGFA gene and protein expression in MF. Methods and results: First, we compared VEGFA mRNA levels in MF and in normal T lymphocyte samples; significantly higher VEGFA levels were found in MF. We then studied VEGFA expression in different normal T cell subsets, focusing on CD4+, CD8+, resting and activated T lymphocytes. We applied the gene signatures of the normal T cell subsets to MF samples and found that activated T lymphocytes represented the closest normal counterpart of the tumour. However, VEGFA mRNA levels were significantly higher in MF than in activated normal T cells, suggesting that VEGFA overexpression in MF represents an attribute acquired during neoplastic transformation: no significant VEGFA expression differences were recorded between early and advanced stages. Gene expression profile results were supported by immunohistochemistry in routine sections from 27 MF cases. Conclusions: For the first time, we demonstrate VEGFA expression in MF cells, suggesting that the VEGF pathway may be implicated in MF pathogenesis and can represent a novel therapeutic target.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalHistopathology
Volume66
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • Immunohistochemistry
  • Microarray
  • Mycosis fungoides
  • VEGFA

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Medicine(all)

Fingerprint Dive into the research topics of 'Vascular endothelial growth factor A (VEGFA) expression in mycosis fungoides'. Together they form a unique fingerprint.

  • Cite this

    Pileri, A., Agostinelli, C., Righi, S., Fuligni, F., Bacci, F., Sabattini, E., Patrizi, A., Pileri, S. A., & Piccaluga, P. P. (2015). Vascular endothelial growth factor A (VEGFA) expression in mycosis fungoides. Histopathology, 66(2), 173-181. https://doi.org/10.1111/his.12445