Vascular endothelial growth factor activities on osteoarthritic chondrocytes

Lia Pulsatelli, Paolo Dolzani, Tania Silvestri, Luigi Frizziero, Andrea Facchini, Riccardo Meliconi

Research output: Contribution to journalArticle

Abstract

Objective: Evaluation of the role of VEGF in cartilage pathophysiology. Methods: VEGF release from chondrocytes in the presence of IL-1β, TGFβ and IL-10 was detected by immunoassay. VEGF receptor -1 and -2 expression and VEGF ability to modulate caspase-3 and cathepsin B expression were detected by immunohistochemistry on cartilage biopsies and cartilage explants. VEGF effects on chondrocyte proliferation was analysed by a fluorescent dye that binds nucleic acids. Results: VEGF production by osteoartritis (OA) chondrocytes was significantly reduced by IL-10 while it was increased in the presence of TGFβ. Cartilage VEGFR-1 immunostaining was significantly downregulated in 'early' OA patients compared to normal controls (NC). VEGFR-2 expression was negligible both in OA and in NC. VEGF decreased the expression of caspase-3 and cathepsin B, whereas it did not affect proliferation. Conclusion: VEGF is able to down-modulate chrondrocyte activities related to catabolic events involved in OA cartilage degradation.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalClinical and Experimental Rheumatology
Volume23
Issue number4
Publication statusPublished - Jul 2005

Keywords

  • Chondrocytes
  • Osteoarthritis
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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