Vascular endothelial growth factor and tryptase changes after chemoembolization in hepatocarcinoma patients

Girolamo Ranieri, Michele Ammendola, Ilaria Marech, Annamaria Laterza, Ines Abbate, Caroline Oakley, Angelo Vacca, Rosario Sacco, Cosmo Damiano Gadaleta

Research output: Contribution to journalArticle

Abstract

AIM: To evaluate vascular endothelial growth factor (VEGF) and tryptase in hepatocellular cancer (HCC) before and after trans-arterial chemoembolization (TACE). METHODS: VEGF and tryptase serum concentrations were assessed from 71 unresectable HCC patients before and after hepatic TACE performed by binding DC-Beads® to doxorubicin. VEGF levels were examined for each serum sample using the Quantikine Human VEGF-enzyme-linked immuno-absorbent assay (ELISA), whereas tryptase serum concentrations were assessed for each serum sample by means of fluoro-enzyme immunoassay (FEIA) using the Uni-CAP100 tool. Differences between serum VEGF and tryptase values before and after TACE were evaluated using Student t test. Person's correlation was used to assess the degree of association between the two variables. RESULTS: VEGF levels and serum tryptase in HCC patients before TACE had a mean value and standard deviation (SD) of 114.31 ± 79.58 pg/mL and 8.13 ± 3.61 ìg/L, respectively. The mean levels and SD of VEGF levels and serum tryptase in HCC patients after TACE were 238.14 ± 109.41 pg/mL and 4.02 ± 3.03 ìg/L. The changes between the mean values of concentration of VEGF and tryptase before treatment and after treatment was statistically significant (P <0.000231 and P <0.00124, by Wilcoxon-Mann-Whitney respectively). A significant correlation between VEGF levels before and after TACE and between tryptase levels before and after TACE was demonstrated (r = 0.68, P = 0.003; r = 0.84, P = 0.000 respectively). CONCLUSION: Our pilot results suggest that the higher serum VEGF levels and the lower tryptase levels following TACE may be potential biomarkers changing in response to therapy.

Original languageEnglish
Pages (from-to)6018-6025
Number of pages8
JournalWorld Journal of Gastroenterology
Volume21
Issue number19
DOIs
Publication statusPublished - May 21 2015

Keywords

  • Chemoembolization
  • Hepatocellular cancer
  • Serum levels
  • Tryptase
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Gastroenterology

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