Vascular endothelial growth factor concentrations in the plasma-activated platelets rich (P-APR) of healthy controls and colorectal cancer patients

Girolamo Ranieri, Maria Coviello, Rosa Patruno, Paolo Valerio, Domenico Martino, Pietro Milella, Vittorio Catalano, Francesco Scotto, Antonella De Ceglie, Michele Quaranta, Domenico Ribatti, Antonio Pellecchia

Research output: Contribution to journalArticle

Abstract

Vascular endothelial growth factor (VEGF) is known to play a key role in tumour angiogenesis. Our preliminary published data suggest that plasma-activated platelets rich (P-APR) rather than other plasma compartments (i.e. plasma, plasma-platelets poor) or serum is the more suitable blood fraction for measuring VEGF in a miscellaneous series of gastrointestinal cancer patients. The aim of this confirmatory study was to assess VEGF in P-APR blood compartments of 30 healthy control subjects (HCS) and a homogeneous series of 62 colorectal cancer patients (CRCP), prospectively collected, to evaluate its possible clinical-biological significance. Samples of plasma (P) in both sodium citrate (SC) and sodium citrate-theophylline-adenosine-dipyridamole (CTAD) were collected from venous blood. After the centrifugation and separation methods VEGF levels were detected by ELISA in P-APR. The best differentiation between HCS and CRCP in VEGF level was seen for P-APRCTAD (median value: 255 pg/ml versus 142 pg/ml; p=0.000 by Mann-Whitney U test). No significant correlation among the P-APR VEGF concentrations and the main clinical pathological features was found. We suggest that P-APRCTAD fraction, obtained according to well standardised conditions, could represent the suitable blood compartment for the assessment of VEGF as marker of malignant intestinal transformation.

Original languageEnglish
Pages (from-to)817-820
Number of pages4
JournalOncology Reports
Volume12
Issue number4
Publication statusPublished - Oct 2004

Keywords

  • Colorectal cancer
  • ELISA
  • Healthy controls
  • Plasma VEGF
  • Plasma-activated platelets rich
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Ranieri, G., Coviello, M., Patruno, R., Valerio, P., Martino, D., Milella, P., Catalano, V., Scotto, F., De Ceglie, A., Quaranta, M., Ribatti, D., & Pellecchia, A. (2004). Vascular endothelial growth factor concentrations in the plasma-activated platelets rich (P-APR) of healthy controls and colorectal cancer patients. Oncology Reports, 12(4), 817-820.