Vascular endothelial growth factors synthesized by human lung mast cells exert angiogenic effects

Aikaterini Detoraki, Rosaria I. Staiano, Francescopaolo Granata, Giorgio Giannattasio, Nella Prevete, Amato de Paulis, Domenico Ribatti, Arturo Genovese, Massimo Triggiani, Gianni Marone

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Background: Angiogenesis and lymphangiogenesis are critical for several allergic, inflammatory, and neoplastic disorders. Mast cells infiltrate the sites of inflammation and tumors. Objective: We sought to characterize the expression and functions of vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) in human mast cells. Methods: VEGF expression was evaluated by means of RT-PCR and Western blotting in primary human lung mast cells and in the mast cell lines LAD-2 and HMC-1. Angiogenic activity of mast cell supernatants was determined by using the chick embryo chorioallantoic membrane assay. VEGFR expression was assessed by means of RT-PCR and flow cytometry. Modified Boyden chambers were used for chemotaxis assay. Results: Human mast cells express VEGF-A, VEGF-B, VEGF-C, and VEGF-D at both the mRNA and protein level. Prostaglandin E2 (PGE2) enhanced the expression of VEGFA, VEGFB, and VEGFC, whereas an adenosine analog (5′-[N-ethylcarboxamido] adenosine [NECA]) increased VEGFA, VEGFC, and VEGFD expression. In addition, PGE2 and NECA enhanced VEGF-A release, and supernatants of PGE2- and NECA-activated human lung mast cells induced angiogenic responses in the chorioallantoic membrane assay that were inhibited by an anti-VEGF-A antibody. Mast cells expressed mRNA for VEGFR1 and VEGFR2. These receptors were present on the mast cell surface. VEGF-A165, VEGF-B167, VEGF-C, VEGF-D, and placental growth factor 1 induced mast cell chemotaxis. These chemotactic effects were mediated by the activation of both VEGFR-1 and VEGFR-2. Conclusion: Our data indicate that human mast cells are both a source and a target of angiogenic and lymphangiogenic factors and therefore might play a role in inflammatory and neoplastic angiogenesis through the expression of several forms of VEGFs and their receptors.

Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Volume123
Issue number5
DOIs
Publication statusPublished - May 2009

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Mast Cells
Lung
Vascular Endothelial Growth Factor Receptor
Vascular Endothelial Growth Factors
Adenosine
Vascular Endothelial Growth Factor D
Dinoprostone
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor C
Chorioallantoic Membrane
Chemotaxis
Vascular Endothelial Growth Factor B
human VEGFA protein
Lymphangiogenesis
Polymerase Chain Reaction
Messenger RNA
Angiogenesis Inducing Agents
Chick Embryo
Intercellular Signaling Peptides and Proteins
Flow Cytometry

Keywords

  • adenosine
  • angiogenesis
  • chemotaxis
  • Human mast cells
  • prostaglandin E
  • vascular endothelial growth factor receptors
  • vascular endothelial growth factors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Detoraki, A., Staiano, R. I., Granata, F., Giannattasio, G., Prevete, N., de Paulis, A., ... Marone, G. (2009). Vascular endothelial growth factors synthesized by human lung mast cells exert angiogenic effects. Journal of Allergy and Clinical Immunology, 123(5). https://doi.org/10.1016/j.jaci.2009.01.044

Vascular endothelial growth factors synthesized by human lung mast cells exert angiogenic effects. / Detoraki, Aikaterini; Staiano, Rosaria I.; Granata, Francescopaolo; Giannattasio, Giorgio; Prevete, Nella; de Paulis, Amato; Ribatti, Domenico; Genovese, Arturo; Triggiani, Massimo; Marone, Gianni.

In: Journal of Allergy and Clinical Immunology, Vol. 123, No. 5, 05.2009.

Research output: Contribution to journalArticle

Detoraki, A, Staiano, RI, Granata, F, Giannattasio, G, Prevete, N, de Paulis, A, Ribatti, D, Genovese, A, Triggiani, M & Marone, G 2009, 'Vascular endothelial growth factors synthesized by human lung mast cells exert angiogenic effects', Journal of Allergy and Clinical Immunology, vol. 123, no. 5. https://doi.org/10.1016/j.jaci.2009.01.044
Detoraki, Aikaterini ; Staiano, Rosaria I. ; Granata, Francescopaolo ; Giannattasio, Giorgio ; Prevete, Nella ; de Paulis, Amato ; Ribatti, Domenico ; Genovese, Arturo ; Triggiani, Massimo ; Marone, Gianni. / Vascular endothelial growth factors synthesized by human lung mast cells exert angiogenic effects. In: Journal of Allergy and Clinical Immunology. 2009 ; Vol. 123, No. 5.
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abstract = "Background: Angiogenesis and lymphangiogenesis are critical for several allergic, inflammatory, and neoplastic disorders. Mast cells infiltrate the sites of inflammation and tumors. Objective: We sought to characterize the expression and functions of vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) in human mast cells. Methods: VEGF expression was evaluated by means of RT-PCR and Western blotting in primary human lung mast cells and in the mast cell lines LAD-2 and HMC-1. Angiogenic activity of mast cell supernatants was determined by using the chick embryo chorioallantoic membrane assay. VEGFR expression was assessed by means of RT-PCR and flow cytometry. Modified Boyden chambers were used for chemotaxis assay. Results: Human mast cells express VEGF-A, VEGF-B, VEGF-C, and VEGF-D at both the mRNA and protein level. Prostaglandin E2 (PGE2) enhanced the expression of VEGFA, VEGFB, and VEGFC, whereas an adenosine analog (5′-[N-ethylcarboxamido] adenosine [NECA]) increased VEGFA, VEGFC, and VEGFD expression. In addition, PGE2 and NECA enhanced VEGF-A release, and supernatants of PGE2- and NECA-activated human lung mast cells induced angiogenic responses in the chorioallantoic membrane assay that were inhibited by an anti-VEGF-A antibody. Mast cells expressed mRNA for VEGFR1 and VEGFR2. These receptors were present on the mast cell surface. VEGF-A165, VEGF-B167, VEGF-C, VEGF-D, and placental growth factor 1 induced mast cell chemotaxis. These chemotactic effects were mediated by the activation of both VEGFR-1 and VEGFR-2. Conclusion: Our data indicate that human mast cells are both a source and a target of angiogenic and lymphangiogenic factors and therefore might play a role in inflammatory and neoplastic angiogenesis through the expression of several forms of VEGFs and their receptors.",
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AU - Detoraki, Aikaterini

AU - Staiano, Rosaria I.

AU - Granata, Francescopaolo

AU - Giannattasio, Giorgio

AU - Prevete, Nella

AU - de Paulis, Amato

AU - Ribatti, Domenico

AU - Genovese, Arturo

AU - Triggiani, Massimo

AU - Marone, Gianni

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N2 - Background: Angiogenesis and lymphangiogenesis are critical for several allergic, inflammatory, and neoplastic disorders. Mast cells infiltrate the sites of inflammation and tumors. Objective: We sought to characterize the expression and functions of vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) in human mast cells. Methods: VEGF expression was evaluated by means of RT-PCR and Western blotting in primary human lung mast cells and in the mast cell lines LAD-2 and HMC-1. Angiogenic activity of mast cell supernatants was determined by using the chick embryo chorioallantoic membrane assay. VEGFR expression was assessed by means of RT-PCR and flow cytometry. Modified Boyden chambers were used for chemotaxis assay. Results: Human mast cells express VEGF-A, VEGF-B, VEGF-C, and VEGF-D at both the mRNA and protein level. Prostaglandin E2 (PGE2) enhanced the expression of VEGFA, VEGFB, and VEGFC, whereas an adenosine analog (5′-[N-ethylcarboxamido] adenosine [NECA]) increased VEGFA, VEGFC, and VEGFD expression. In addition, PGE2 and NECA enhanced VEGF-A release, and supernatants of PGE2- and NECA-activated human lung mast cells induced angiogenic responses in the chorioallantoic membrane assay that were inhibited by an anti-VEGF-A antibody. Mast cells expressed mRNA for VEGFR1 and VEGFR2. These receptors were present on the mast cell surface. VEGF-A165, VEGF-B167, VEGF-C, VEGF-D, and placental growth factor 1 induced mast cell chemotaxis. These chemotactic effects were mediated by the activation of both VEGFR-1 and VEGFR-2. Conclusion: Our data indicate that human mast cells are both a source and a target of angiogenic and lymphangiogenic factors and therefore might play a role in inflammatory and neoplastic angiogenesis through the expression of several forms of VEGFs and their receptors.

AB - Background: Angiogenesis and lymphangiogenesis are critical for several allergic, inflammatory, and neoplastic disorders. Mast cells infiltrate the sites of inflammation and tumors. Objective: We sought to characterize the expression and functions of vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) in human mast cells. Methods: VEGF expression was evaluated by means of RT-PCR and Western blotting in primary human lung mast cells and in the mast cell lines LAD-2 and HMC-1. Angiogenic activity of mast cell supernatants was determined by using the chick embryo chorioallantoic membrane assay. VEGFR expression was assessed by means of RT-PCR and flow cytometry. Modified Boyden chambers were used for chemotaxis assay. Results: Human mast cells express VEGF-A, VEGF-B, VEGF-C, and VEGF-D at both the mRNA and protein level. Prostaglandin E2 (PGE2) enhanced the expression of VEGFA, VEGFB, and VEGFC, whereas an adenosine analog (5′-[N-ethylcarboxamido] adenosine [NECA]) increased VEGFA, VEGFC, and VEGFD expression. In addition, PGE2 and NECA enhanced VEGF-A release, and supernatants of PGE2- and NECA-activated human lung mast cells induced angiogenic responses in the chorioallantoic membrane assay that were inhibited by an anti-VEGF-A antibody. Mast cells expressed mRNA for VEGFR1 and VEGFR2. These receptors were present on the mast cell surface. VEGF-A165, VEGF-B167, VEGF-C, VEGF-D, and placental growth factor 1 induced mast cell chemotaxis. These chemotactic effects were mediated by the activation of both VEGFR-1 and VEGFR-2. Conclusion: Our data indicate that human mast cells are both a source and a target of angiogenic and lymphangiogenic factors and therefore might play a role in inflammatory and neoplastic angiogenesis through the expression of several forms of VEGFs and their receptors.

KW - adenosine

KW - angiogenesis

KW - chemotaxis

KW - Human mast cells

KW - prostaglandin E

KW - vascular endothelial growth factor receptors

KW - vascular endothelial growth factors

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