Vasomotor effects of hydrogen sulfide in human umbilical vessels

R Mohammed, L Provitera, G Cavallaro, D Lattuada, G Ercoli, F Mosca, E Villamor

Research output: Contribution to journalArticle

Abstract

Hydrogen sulfide (H2S) has recently emerged as a biologically active gas with multiple effects on the cardiovascular system. We aimed to investigate the vasomotor actions of sodium sulfide (Na2S), which forms H2S and HS- in solution, in human umbilical artery (HUA) and vein (HUV) rings. In addition, we examined by immunocytochemistry the expression and localization of cystathionine β-synthase (CBS), cystathionine lyase (CSE), and 3-mercaptopyruvate sulphurtransferase (MPST), the enzymes responsible for endogenous H2S production. Human umbilical vessels were compared with chicken embryo umbilical vessels. HUA and HUV expressed a robust signal for CSE, CBS, and 3-MPST in both endothelial and smooth muscle cells. However, HUA rings did not respond to Na2S (10-6M-10-3M) either at resting tone or during contraction evoked by serotonin or KCl. Similarly, the extraembryonic part of chicken allantoic artery did not respond to Na2S. In contrast, Na2S induced a concentration-dependent contraction in HUV rings under resting tone and a concentration-dependent relaxation when the H2UV rings were contracted with serotonin (42 ± 5% relaxation) or KCl (12 ± 5% relaxation). Na2S-induced contraction of HUV was impaired following removal of extracellular Ca2+, endothelial denudation, NO synthase inhibition (L-NAME), or soluble guanylate cyclase (sGC) inhibition (ODQ). Na2S-induced relaxation of HUV was impaired by the KATP channel inhibitor glibenclamide. In conclusion, H2S does not have vasomotor effects on HUA but induced contraction (mediated through inactivation of the NO/sGC axis) and relaxation (mediated through KATP channels) in HUV. Our data suggest a role for H2S in the venous side of human umbilical circulation.

Original languageEnglish
Pages (from-to)737-747
Number of pages11
JournalJournal of Physiology and Pharmacology
Volume68
Issue number5
Publication statusPublished - Oct 2017

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Umbilicus
Hydrogen Sulfide
Umbilical Arteries
Cystathionine
Veins
KATP Channels
Umbilical Veins
Lyases
Chickens
Serotonin
Glyburide
NG-Nitroarginine Methyl Ester
Cardiovascular System
sodium sulfide
Nitric Oxide Synthase
Smooth Muscle Myocytes
Embryonic Structures
Arteries
Gases
Immunohistochemistry

Keywords

  • Journal Article

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Vasomotor effects of hydrogen sulfide in human umbilical vessels. / Mohammed, R; Provitera, L; Cavallaro, G; Lattuada, D; Ercoli, G; Mosca, F; Villamor, E.

In: Journal of Physiology and Pharmacology, Vol. 68, No. 5, 10.2017, p. 737-747.

Research output: Contribution to journalArticle

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