An in vitro isolated working frog heart (Rana esculenta) was used to study the effects of exogenous CGA1-76 (vasostatin 1), CGA1-113 (vasostatin 2), and the synthetic CGA7-57 on cardiac performance. Under basal cardiac conditions, the dose-response curves of the three peptides from 10-8 to 10-7 M showed a significant calcium-dependent negative inotropism that involved neither the endocardial endothelium nor the adrenergic and muscarinic receptors. In addition, the CgA fragments clearly counteracted the typical positive inotropism of isoprenaline (10-9 M). Taken together, these results provide the first evidence for a cardio-suppressive role for the vasostatins.
|Number of pages||4|
|Journal||Annals of the New York Academy of Sciences|
|Publication status||Published - Jun 5 2002|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)