Vav binding to heterogeneous nuclear ribonucleoprotein (hnRNP) C

Evidence for Vav-hnRNP interactions in an RNA-dependent manner

Francisco Romero, Antonia Germani, Edmond Puvion, Jacques Camonis, Nadine Varin-Blank, Sylvie Gisselbrecht, Siegmund Fischer

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The vav proto-oncogene is exclusively expressed in hematopoietic cells and encodes a 95-kDa protein that contains multiple structural domains. Vav is involved in the expansion of T and B cells, in antigen-mediated proliferative responses, and in the induction of intrathymic T cell maturation. It becomes rapidly and transiently tyrosine-phosphorylated upon triggering of a large number of surface receptors and catalyzes GDP/GTP exchange on Rac-1. We now provide evidence for the specific interaction of Vav with heterogeneous nuclear ribonucleoprotein (hnRNP) C. Vav and hnRNP C interact both in vive and in vitro mediated through the carboxyl Src homology 3 domain of Vav and the proline-rich motif located in the nuclear retention sequence of hnRNP C. More importantly, Vav-hnRNP C complexes are present in living hematopoietic cells and both proteins localize in the nuclei, mainly on perichromatic fibrils but also on clusters of interchromatin granules. The Vav-hnRNP C interaction is regulated by poly(U) RNA, although a basal association is still detected in the absence of RNA. Furthermore, RNA homopolymers differentially alter the binding affinity of Vav to hnRNP C and hnRNP K. We propose that Vav-hnRNP interactions may be established in an RNA- dependent manner.

Original languageEnglish
Pages (from-to)5923-5931
Number of pages9
JournalJournal of Biological Chemistry
Volume273
Issue number10
DOIs
Publication statusPublished - Mar 6 1998

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Heterogeneous-Nuclear Ribonucleoprotein Group C
Heterogeneous-Nuclear Ribonucleoproteins
RNA
Heterogeneous-Nuclear Ribonucleoprotein K
T-Lymphocytes
Poly U
src Homology Domains
T-cells
Proto-Oncogenes
Guanosine Triphosphate
Homopolymerization
Proline
Tyrosine
Proteins
B-Lymphocytes
Cells
Association reactions
Antigens

ASJC Scopus subject areas

  • Biochemistry

Cite this

Vav binding to heterogeneous nuclear ribonucleoprotein (hnRNP) C : Evidence for Vav-hnRNP interactions in an RNA-dependent manner. / Romero, Francisco; Germani, Antonia; Puvion, Edmond; Camonis, Jacques; Varin-Blank, Nadine; Gisselbrecht, Sylvie; Fischer, Siegmund.

In: Journal of Biological Chemistry, Vol. 273, No. 10, 06.03.1998, p. 5923-5931.

Research output: Contribution to journalArticle

Romero, F, Germani, A, Puvion, E, Camonis, J, Varin-Blank, N, Gisselbrecht, S & Fischer, S 1998, 'Vav binding to heterogeneous nuclear ribonucleoprotein (hnRNP) C: Evidence for Vav-hnRNP interactions in an RNA-dependent manner', Journal of Biological Chemistry, vol. 273, no. 10, pp. 5923-5931. https://doi.org/10.1074/jbc.273.10.5923
Romero, Francisco ; Germani, Antonia ; Puvion, Edmond ; Camonis, Jacques ; Varin-Blank, Nadine ; Gisselbrecht, Sylvie ; Fischer, Siegmund. / Vav binding to heterogeneous nuclear ribonucleoprotein (hnRNP) C : Evidence for Vav-hnRNP interactions in an RNA-dependent manner. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 10. pp. 5923-5931.
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