Vav1 is a component of transcriptionally active complexes

Martin Houlard, Ramachandran Arudchandran, Fabienne Regnier-Ricard, Antonia Germani, Sylvie Gisselbrecht, Ulrich Blank, Juan Rivera, Nadine Varin-Blank

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The importance of the hematopoietic protooncogene Vav1 in immune cell function is widely recognized, although its regulatory mechanisms are not completely understood. Here, we examined whether Vav1 has a nuclear function, as past studies have reported its nuclear localization. Our findings provide a definitive demonstration of Vav1 nuclear localization in a receptor stimulation-dependent manner and reveal a critical role for the COOH-terminal Src homology 3 (SH3) domain and a nuclear localization sequence within the pleckstrin homology domain. Analysis of DNA-bound transcription factor complexes revealed nuclear Vav1 as an integral component of transcriptionally active nuclear factor of activated T cells (NFAT)- and nuclear factor (NF)KB-like complexes, and the COOH-terminal SH3 domain as being critical in their formation. Thus, we describe a novel nuclear role for Vav1 as a component and facilitator of NFAT and NFKB-like transcriptional activity.

Original languageEnglish
Pages (from-to)1115-1127
Number of pages13
JournalJournal of Experimental Medicine
Volume195
Issue number9
DOIs
Publication statusPublished - May 6 2002

Fingerprint

NFATC Transcription Factors
src Homology Domains
TCF Transcription Factors
Transcription Factors
DNA
Pleckstrin Homology Domains

Keywords

  • Calcium influx
  • Nuclear factor of activated T cells
  • Nuclear translocation
  • Protein subdomains
  • Vav

ASJC Scopus subject areas

  • Immunology

Cite this

Houlard, M., Arudchandran, R., Regnier-Ricard, F., Germani, A., Gisselbrecht, S., Blank, U., ... Varin-Blank, N. (2002). Vav1 is a component of transcriptionally active complexes. Journal of Experimental Medicine, 195(9), 1115-1127. https://doi.org/10.1084/jem.20011701

Vav1 is a component of transcriptionally active complexes. / Houlard, Martin; Arudchandran, Ramachandran; Regnier-Ricard, Fabienne; Germani, Antonia; Gisselbrecht, Sylvie; Blank, Ulrich; Rivera, Juan; Varin-Blank, Nadine.

In: Journal of Experimental Medicine, Vol. 195, No. 9, 06.05.2002, p. 1115-1127.

Research output: Contribution to journalArticle

Houlard, M, Arudchandran, R, Regnier-Ricard, F, Germani, A, Gisselbrecht, S, Blank, U, Rivera, J & Varin-Blank, N 2002, 'Vav1 is a component of transcriptionally active complexes', Journal of Experimental Medicine, vol. 195, no. 9, pp. 1115-1127. https://doi.org/10.1084/jem.20011701
Houlard M, Arudchandran R, Regnier-Ricard F, Germani A, Gisselbrecht S, Blank U et al. Vav1 is a component of transcriptionally active complexes. Journal of Experimental Medicine. 2002 May 6;195(9):1115-1127. https://doi.org/10.1084/jem.20011701
Houlard, Martin ; Arudchandran, Ramachandran ; Regnier-Ricard, Fabienne ; Germani, Antonia ; Gisselbrecht, Sylvie ; Blank, Ulrich ; Rivera, Juan ; Varin-Blank, Nadine. / Vav1 is a component of transcriptionally active complexes. In: Journal of Experimental Medicine. 2002 ; Vol. 195, No. 9. pp. 1115-1127.
@article{cc837a2ba22e4d2d97ad2a9a611ae7b8,
title = "Vav1 is a component of transcriptionally active complexes",
abstract = "The importance of the hematopoietic protooncogene Vav1 in immune cell function is widely recognized, although its regulatory mechanisms are not completely understood. Here, we examined whether Vav1 has a nuclear function, as past studies have reported its nuclear localization. Our findings provide a definitive demonstration of Vav1 nuclear localization in a receptor stimulation-dependent manner and reveal a critical role for the COOH-terminal Src homology 3 (SH3) domain and a nuclear localization sequence within the pleckstrin homology domain. Analysis of DNA-bound transcription factor complexes revealed nuclear Vav1 as an integral component of transcriptionally active nuclear factor of activated T cells (NFAT)- and nuclear factor (NF)KB-like complexes, and the COOH-terminal SH3 domain as being critical in their formation. Thus, we describe a novel nuclear role for Vav1 as a component and facilitator of NFAT and NFKB-like transcriptional activity.",
keywords = "Calcium influx, Nuclear factor of activated T cells, Nuclear translocation, Protein subdomains, Vav",
author = "Martin Houlard and Ramachandran Arudchandran and Fabienne Regnier-Ricard and Antonia Germani and Sylvie Gisselbrecht and Ulrich Blank and Juan Rivera and Nadine Varin-Blank",
year = "2002",
month = "5",
day = "6",
doi = "10.1084/jem.20011701",
language = "English",
volume = "195",
pages = "1115--1127",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "9",

}

TY - JOUR

T1 - Vav1 is a component of transcriptionally active complexes

AU - Houlard, Martin

AU - Arudchandran, Ramachandran

AU - Regnier-Ricard, Fabienne

AU - Germani, Antonia

AU - Gisselbrecht, Sylvie

AU - Blank, Ulrich

AU - Rivera, Juan

AU - Varin-Blank, Nadine

PY - 2002/5/6

Y1 - 2002/5/6

N2 - The importance of the hematopoietic protooncogene Vav1 in immune cell function is widely recognized, although its regulatory mechanisms are not completely understood. Here, we examined whether Vav1 has a nuclear function, as past studies have reported its nuclear localization. Our findings provide a definitive demonstration of Vav1 nuclear localization in a receptor stimulation-dependent manner and reveal a critical role for the COOH-terminal Src homology 3 (SH3) domain and a nuclear localization sequence within the pleckstrin homology domain. Analysis of DNA-bound transcription factor complexes revealed nuclear Vav1 as an integral component of transcriptionally active nuclear factor of activated T cells (NFAT)- and nuclear factor (NF)KB-like complexes, and the COOH-terminal SH3 domain as being critical in their formation. Thus, we describe a novel nuclear role for Vav1 as a component and facilitator of NFAT and NFKB-like transcriptional activity.

AB - The importance of the hematopoietic protooncogene Vav1 in immune cell function is widely recognized, although its regulatory mechanisms are not completely understood. Here, we examined whether Vav1 has a nuclear function, as past studies have reported its nuclear localization. Our findings provide a definitive demonstration of Vav1 nuclear localization in a receptor stimulation-dependent manner and reveal a critical role for the COOH-terminal Src homology 3 (SH3) domain and a nuclear localization sequence within the pleckstrin homology domain. Analysis of DNA-bound transcription factor complexes revealed nuclear Vav1 as an integral component of transcriptionally active nuclear factor of activated T cells (NFAT)- and nuclear factor (NF)KB-like complexes, and the COOH-terminal SH3 domain as being critical in their formation. Thus, we describe a novel nuclear role for Vav1 as a component and facilitator of NFAT and NFKB-like transcriptional activity.

KW - Calcium influx

KW - Nuclear factor of activated T cells

KW - Nuclear translocation

KW - Protein subdomains

KW - Vav

UR - http://www.scopus.com/inward/record.url?scp=0037029646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037029646&partnerID=8YFLogxK

U2 - 10.1084/jem.20011701

DO - 10.1084/jem.20011701

M3 - Article

VL - 195

SP - 1115

EP - 1127

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 9

ER -