Vav1 is a component of transcriptionally active complexes

Martin Houlard, Ramachandran Arudchandran, Fabienne Regnier-Ricard, Antonia Germani, Sylvie Gisselbrecht, Ulrich Blank, Juan Rivera, Nadine Varin-Blank

Research output: Contribution to journalArticle

Abstract

The importance of the hematopoietic protooncogene Vav1 in immune cell function is widely recognized, although its regulatory mechanisms are not completely understood. Here, we examined whether Vav1 has a nuclear function, as past studies have reported its nuclear localization. Our findings provide a definitive demonstration of Vav1 nuclear localization in a receptor stimulation-dependent manner and reveal a critical role for the COOH-terminal Src homology 3 (SH3) domain and a nuclear localization sequence within the pleckstrin homology domain. Analysis of DNA-bound transcription factor complexes revealed nuclear Vav1 as an integral component of transcriptionally active nuclear factor of activated T cells (NFAT)- and nuclear factor (NF)KB-like complexes, and the COOH-terminal SH3 domain as being critical in their formation. Thus, we describe a novel nuclear role for Vav1 as a component and facilitator of NFAT and NFKB-like transcriptional activity.

Original languageEnglish
Pages (from-to)1115-1127
Number of pages13
JournalJournal of Experimental Medicine
Volume195
Issue number9
DOIs
Publication statusPublished - May 6 2002

Keywords

  • Calcium influx
  • Nuclear factor of activated T cells
  • Nuclear translocation
  • Protein subdomains
  • Vav

ASJC Scopus subject areas

  • Immunology

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    Houlard, M., Arudchandran, R., Regnier-Ricard, F., Germani, A., Gisselbrecht, S., Blank, U., Rivera, J., & Varin-Blank, N. (2002). Vav1 is a component of transcriptionally active complexes. Journal of Experimental Medicine, 195(9), 1115-1127. https://doi.org/10.1084/jem.20011701