Ventricular myosin and creatine-kinase isoenzymes in hypertensive rats treated with captopril

P. Pauletto, L. Nascimben, D. Piccolo, S. Secchiero, G. Vescovo, G. Scannapieco, L. Dalla Libera, U. Carraro, A. C. Pessina, C. Dal Palu

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Abstract

In the myocardium, myosin and creatine kinase isoforms possess different capacities for using O2 and energy-rich phosphates. We studied electrophoretically the distribution of these isoforms in 19 hypertensive rats (two-kidney, one clip model of hypertension) and in age-matched controls. After 6 weeks of hypertension, seven rats were treated with captopril (2 mg/kg daily) for 4 weeks, six were left hypertensive for another 4 weeks, and the remaining rats were killed under ether anesthesia. In the latter, ventricular mass was significantly higher than in controls; V3 isomyosin was 32.3±6.8% versus 0%, and both creatine kinase-MB and -BB were increased at the expense of creatine kinase-MM (creatine kinase-MB=29±2.8% vs. 14.7±1.8%, p3 isomyosin, and both creatine kinase-MB and -BB isoforms were fond to be persistently higher than in controls. At the same time, captopril-treated rats showed reduced but not normalized blood pressure levels, normalized ventricular mass, and prevalence of the V1 isomyosin (56.9±22% vs. 47.9±23.8% in normotensive controls, p=NS). However, higher levels of creatine kinase-MB and -BB were still found in these rats in comparison with the normotensive controls (creatine kinase-MB=22.4±5.4% vs. 15.8±2.8%, p

Original languageEnglish
Pages (from-to)556-562
Number of pages7
JournalHypertension
Volume14
Issue number5
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Internal Medicine

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    Pauletto, P., Nascimben, L., Piccolo, D., Secchiero, S., Vescovo, G., Scannapieco, G., Dalla Libera, L., Carraro, U., Pessina, A. C., & Dal Palu, C. (1989). Ventricular myosin and creatine-kinase isoenzymes in hypertensive rats treated with captopril. Hypertension, 14(5), 556-562.