Verapamil upregulates sensitivity of human colon and breast cancer cells to LAK-cytotoxicity in vitro

Pierpaolo Correale, Pierosandro Tagliaferri, Luigi Celio, Geppino Genua, Stefania Montagnani, Angelo Raffaele Bianco

Research output: Contribution to journalArticle

Abstract

Pretreatment of human colon cancer LoVo-H cells and human breast cancer ZR-75 1A cells with low doses of verapamil, a Ca2+ channel blocker, for 48 h has a slight growth stimulatory effect and substantially increases cell sensitivity to lymphokine-activated killer (LAK) mediated cytotoxicity in the standard 51Cr release assay. The role of intracellular Ca2+ levels in determining verapamil effect is demonstrated by cytochemical evidence of intracellular Ca2+ lowering in verapamil-treated cells and by the reversal by the Ca2+ ionophore A-23187 of verapamil-induced sensitivity to LAK-mediated cytotoxicity.

Original languageEnglish
Pages (from-to)1393-1395
Number of pages3
JournalEuropean Journal of Cancer and Clinical Oncology
Volume27
Issue number11
DOIs
Publication statusPublished - 1991

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Lymphokine-Activated Killer Cells
Verapamil
Colonic Neoplasms
Up-Regulation
Breast Neoplasms
Lymphokines
Ionophores
Calcimycin
In Vitro Techniques
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Verapamil upregulates sensitivity of human colon and breast cancer cells to LAK-cytotoxicity in vitro. / Correale, Pierpaolo; Tagliaferri, Pierosandro; Celio, Luigi; Genua, Geppino; Montagnani, Stefania; Bianco, Angelo Raffaele.

In: European Journal of Cancer and Clinical Oncology, Vol. 27, No. 11, 1991, p. 1393-1395.

Research output: Contribution to journalArticle

Correale, Pierpaolo ; Tagliaferri, Pierosandro ; Celio, Luigi ; Genua, Geppino ; Montagnani, Stefania ; Bianco, Angelo Raffaele. / Verapamil upregulates sensitivity of human colon and breast cancer cells to LAK-cytotoxicity in vitro. In: European Journal of Cancer and Clinical Oncology. 1991 ; Vol. 27, No. 11. pp. 1393-1395.
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AU - Montagnani, Stefania

AU - Bianco, Angelo Raffaele

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