TY - JOUR
T1 - Very-KIND is a novel nervous system specific guanine nucleotide exchange factor for Ras GTPases
AU - Mees, Anaid
AU - Rock, Rebecca
AU - Ciccarelli, Francesca D.
AU - Leberfinger, Cornelia B.
AU - Borawski, Johanna M.
AU - Bork, Peer
AU - Wiese, Stefan
AU - Gessler, Manfred
AU - Kerkhoff, Eugen
PY - 2005/12
Y1 - 2005/12
N2 - The kinase non-catalytic c-lobe domain (KIND) evolved from the catalytic protein kinase fold into a potential protein interaction module for signalling proteins. Spir family actin organizers and the non-receptor phosphatase type 13 (PTP type 13) encode a KIND domain in the very N-terminal parts of the proteins. Here we report the characterization and cloning of a third member of the KIND protein family, which we have named very-KIND (VKIND) because of its two KIND domains. Like the other members of the protein family, VKIND has a KIND domain at the N-terminus. A second KIND domain is located in the central part of the protein. The C-terminal half encodes a guanine nucleotide exchange factor motif for Ras-like GTPases (RasGEF) and a RasGEF N-terminal module (RasGEFN). There is only one VKIND gene in the mammalian genomes and up to now we have found the gene only in vertebrates. During mouse embryogenesis the VKIND gene was specifically expressed in the developing nervous system. In adult mice Northern hybridizations revealed high expression only in brain. Low expression could be detected in ovary. In situ hybridizations showed a specific expression of VKIND in neuronal cells of the granular and Purkinje cell layers of the cerebellum.
AB - The kinase non-catalytic c-lobe domain (KIND) evolved from the catalytic protein kinase fold into a potential protein interaction module for signalling proteins. Spir family actin organizers and the non-receptor phosphatase type 13 (PTP type 13) encode a KIND domain in the very N-terminal parts of the proteins. Here we report the characterization and cloning of a third member of the KIND protein family, which we have named very-KIND (VKIND) because of its two KIND domains. Like the other members of the protein family, VKIND has a KIND domain at the N-terminus. A second KIND domain is located in the central part of the protein. The C-terminal half encodes a guanine nucleotide exchange factor motif for Ras-like GTPases (RasGEF) and a RasGEF N-terminal module (RasGEFN). There is only one VKIND gene in the mammalian genomes and up to now we have found the gene only in vertebrates. During mouse embryogenesis the VKIND gene was specifically expressed in the developing nervous system. In adult mice Northern hybridizations revealed high expression only in brain. Low expression could be detected in ovary. In situ hybridizations showed a specific expression of VKIND in neuronal cells of the granular and Purkinje cell layers of the cerebellum.
KW - Cerebellum
KW - KIND
KW - Nervous system
KW - Ras
KW - RasGEF
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=29244462830&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29244462830&partnerID=8YFLogxK
U2 - 10.1016/j.modgep.2005.04.015
DO - 10.1016/j.modgep.2005.04.015
M3 - Article
C2 - 16099729
AN - SCOPUS:29244462830
VL - 6
SP - 79
EP - 85
JO - Brain research. Gene expression patterns
JF - Brain research. Gene expression patterns
SN - 1567-133X
IS - 1
ER -