Viability of olfactory ensheathing cells after hypoxia and serum deprivation: Implication for therapeutic transplantation

Rosalia Pellitteri, Maria Vincenza Catania, Carmela Maria Bonaccorso, Eugenia Ranno, Paola Dell'Albani, Damiano Zaccheo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Olfactory ensheathing cells (OECs) represent glial cells supporting neuronal turnover in the olfactory system. In vitro, OECs promote axonal growth as a source of neurotrophic growth factors; in vivo, they produce myelin, promoting remyelination of damaged axons. Consequently, OEC transplantation appears to be a promising treatment for spinal cord injury, although the functional recovery is limited. This might be ascribed to the microenvironment at the lesion site, lacking growth factors (GFs), nutrients, and oxygen. To mimic this condition, we used an in vitro approach by growing primary neonatal mouse OECs under hypoxic conditions and/or serum deprivation. In addition, we compared OECs survival/proliferation with that of primary cultures of Schwann cells (SCs) and astrocytes under the same experimental conditions. Cultures were analyzed by immunocytochemistry, and cell viability was evaluated by MTT assay. Different GFs, such as NGF, bFGF, and GDNF, and their combination were used to rescue cells from serum and/or oxygen deprivation. We show that the cell types were differently sensitive to the tested stress conditions and that OECs were the most sensitive among them. Moreover, OEC viability was rescued by bFGF under serum-deprived or hypoxic condition but not under conditions of drastic serum deprivation and hypoxia. bFGF was effective also for the other cell types, whereas the effect of the other GFs was negligible. This model suggests that administration of bFGF might be considered useful to sustain cell survival/proliferation after transplantation of OECs either alone or in combination with other glial cell types.

Original languageEnglish
Pages (from-to)1757-1766
Number of pages10
JournalJournal of Neuroscience Research
Volume92
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

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Cell Hypoxia
Transplantation
Serum
Cell Survival
Intercellular Signaling Peptides and Proteins
Therapeutics
Neuroglia
Cell Proliferation
Oxygen
Glial Cell Line-Derived Neurotrophic Factor
Schwann Cells
Cell Transplantation
Nerve Growth Factors
Nerve Growth Factor
Myelin Sheath
Spinal Cord Injuries
Astrocytes
Axons
Immunohistochemistry
Food

Keywords

  • Glial cells
  • Hypoxia
  • Neuroprotection
  • Neurotrophic factors
  • Olfactory bulb

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Viability of olfactory ensheathing cells after hypoxia and serum deprivation : Implication for therapeutic transplantation. / Pellitteri, Rosalia; Catania, Maria Vincenza; Bonaccorso, Carmela Maria; Ranno, Eugenia; Dell'Albani, Paola; Zaccheo, Damiano.

In: Journal of Neuroscience Research, Vol. 92, No. 12, 01.12.2014, p. 1757-1766.

Research output: Contribution to journalArticle

Pellitteri, Rosalia ; Catania, Maria Vincenza ; Bonaccorso, Carmela Maria ; Ranno, Eugenia ; Dell'Albani, Paola ; Zaccheo, Damiano. / Viability of olfactory ensheathing cells after hypoxia and serum deprivation : Implication for therapeutic transplantation. In: Journal of Neuroscience Research. 2014 ; Vol. 92, No. 12. pp. 1757-1766.
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