Viral childhood diarrhoea in Rome: a diagnostic and epidemiological study.

G. Donelli, F. Superti, A. Tinari, M. L. Marziano, D. Caione, C. Concato, D. Menichella

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

During the period May 1987-January 1989, faecal samples from 417 paediatric inpatients admitted to the main paediatric hospital in Rome were screened by direct electron microscopy and rotavirus enzyme-linked immunosorbent assay. Rotaviruses were detected in 18.2% of cases and adenoviruses in 7%, whereas astroviruses were found in 1% of cases. Different percentages of rotavirus excretors were revealed by enzyme-linked immunosorbent assay and electron microscopy. This discrepancy seems to be due to false positive results introduced by enzyme-linked immunosorbent assay. Analysis of electron microscopy-positive samples by rotaviral RNA polyacrylamide gel electrophoresis showed different electropherotypes of rotavirus among which a single, largely predominant long electropherotype (55.4%) was revealed. Short electropherotype subgroup I rotaviruses were demonstrated in about 10.7% of samples.

Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalNew Microbiologica
Volume16
Issue number3
Publication statusPublished - Jul 1993

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Rotavirus
Epidemiologic Studies
Diarrhea
Electron Microscopy
Enzyme-Linked Immunosorbent Assay
Pediatric Hospitals
Adenoviridae
Inpatients
Polyacrylamide Gel Electrophoresis
RNA
Pediatrics

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Donelli, G., Superti, F., Tinari, A., Marziano, M. L., Caione, D., Concato, C., & Menichella, D. (1993). Viral childhood diarrhoea in Rome: a diagnostic and epidemiological study. New Microbiologica, 16(3), 215-225.

Viral childhood diarrhoea in Rome : a diagnostic and epidemiological study. / Donelli, G.; Superti, F.; Tinari, A.; Marziano, M. L.; Caione, D.; Concato, C.; Menichella, D.

In: New Microbiologica, Vol. 16, No. 3, 07.1993, p. 215-225.

Research output: Contribution to journalArticle

Donelli, G, Superti, F, Tinari, A, Marziano, ML, Caione, D, Concato, C & Menichella, D 1993, 'Viral childhood diarrhoea in Rome: a diagnostic and epidemiological study.', New Microbiologica, vol. 16, no. 3, pp. 215-225.
Donelli G, Superti F, Tinari A, Marziano ML, Caione D, Concato C et al. Viral childhood diarrhoea in Rome: a diagnostic and epidemiological study. New Microbiologica. 1993 Jul;16(3):215-225.
Donelli, G. ; Superti, F. ; Tinari, A. ; Marziano, M. L. ; Caione, D. ; Concato, C. ; Menichella, D. / Viral childhood diarrhoea in Rome : a diagnostic and epidemiological study. In: New Microbiologica. 1993 ; Vol. 16, No. 3. pp. 215-225.
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