Abstract
SUMO (small ubiquitin-related modifier) is a ubiquitin-like family member that is conjugated to its substrates through discrete enzymatic steps: activation, involving the E1 enzyme [SAE (SUMO-activating enzyme) 1-SAE2], conjugation, involving the E2 enzyme [Ubc9 (ubiquitin-conjugating enzyme 9)], and substrate modification, through the co-operation of Ubc9 and E3 protein ligases. Work from our laboratory has shown the first example of a viral protein, Gam1, that binds to the E1 heterodimer, inhibiting its function and causing a complete block of the SUMOylation pathway both in vivo and in vitro, followed by SAE1-SAE2 degradation. The mechanism by which a viral protein inactivates and subsequently degrades an essential cellular enzyme, arresting a key regulatory pathway, will be discussed. Although four distinct SUMO isoforms have been described, I will use SUMO to describe the entire system.
| Original language | English |
|---|---|
| Pages (from-to) | 1419-1421 |
| Number of pages | 3 |
| Journal | Biochemical Society Transactions |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- E1 activating enzyme
- Elongin (Elo)
- Gam1
- Small ubiquitin-related modifier (SUMO)
- SUMOylation
- Viral protein
ASJC Scopus subject areas
- Biochemistry
Cite this
Viral control of the SUMO pathway : Gam1, a model system. / Chiocca, S.
In: Biochemical Society Transactions, Vol. 35, No. 6, 12.2007, p. 1419-1421.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Viral control of the SUMO pathway
T2 - Gam1, a model system
AU - Chiocca, S.
PY - 2007/12
Y1 - 2007/12
N2 - SUMO (small ubiquitin-related modifier) is a ubiquitin-like family member that is conjugated to its substrates through discrete enzymatic steps: activation, involving the E1 enzyme [SAE (SUMO-activating enzyme) 1-SAE2], conjugation, involving the E2 enzyme [Ubc9 (ubiquitin-conjugating enzyme 9)], and substrate modification, through the co-operation of Ubc9 and E3 protein ligases. Work from our laboratory has shown the first example of a viral protein, Gam1, that binds to the E1 heterodimer, inhibiting its function and causing a complete block of the SUMOylation pathway both in vivo and in vitro, followed by SAE1-SAE2 degradation. The mechanism by which a viral protein inactivates and subsequently degrades an essential cellular enzyme, arresting a key regulatory pathway, will be discussed. Although four distinct SUMO isoforms have been described, I will use SUMO to describe the entire system.
AB - SUMO (small ubiquitin-related modifier) is a ubiquitin-like family member that is conjugated to its substrates through discrete enzymatic steps: activation, involving the E1 enzyme [SAE (SUMO-activating enzyme) 1-SAE2], conjugation, involving the E2 enzyme [Ubc9 (ubiquitin-conjugating enzyme 9)], and substrate modification, through the co-operation of Ubc9 and E3 protein ligases. Work from our laboratory has shown the first example of a viral protein, Gam1, that binds to the E1 heterodimer, inhibiting its function and causing a complete block of the SUMOylation pathway both in vivo and in vitro, followed by SAE1-SAE2 degradation. The mechanism by which a viral protein inactivates and subsequently degrades an essential cellular enzyme, arresting a key regulatory pathway, will be discussed. Although four distinct SUMO isoforms have been described, I will use SUMO to describe the entire system.
KW - E1 activating enzyme
KW - Elongin (Elo)
KW - Gam1
KW - Small ubiquitin-related modifier (SUMO)
KW - SUMOylation
KW - Viral protein
UR - http://www.scopus.com/inward/record.url?scp=37749041985&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37749041985&partnerID=8YFLogxK
U2 - 10.1042/BST0351419
DO - 10.1042/BST0351419
M3 - Article
C2 - 18031235
AN - SCOPUS:37749041985
VL - 35
SP - 1419
EP - 1421
JO - Biochemical Society Transactions
JF - Biochemical Society Transactions
SN - 0300-5127
IS - 6
ER -