Stool specimens from 127 infants and young children with acute gastroenteritis, aged 2 months-2 years, were examined for detection of rotavirus antigens and particles, and for rotavirus isolation in cell cultures. Paired sera from all the patients were examined by rotavirus serology. Rotavirus particles were demonstrated by direct and/or immune electron microscopy in 58 cases (45.6%), rotavirus antigens were detected by double-antibody sandwich ELISA in feces from 53 cases (41,7%), whereas 40 cases (31,4%) were positive for rotavirus isolation in cell cultures. Sixty-four patients (50,3%) seroconverted by indirect immunoperoxidase and ELISA, including all the cases (58) positive for rotavirus detection in stools and 6 additional cases. In addition, viral agents other than rotavirus possibly responsible for a few cases of gastroenteritis, were detected by electron microscopy in several fecal extracts: adenoviruses (3,9%), enteric coronaviruses (18,8%), small round viruses (1,5%), and astroviruses (1,5%), single or associated each other or with rotaviruses.
|Translated title of the contribution||Viral diagnosis of infantile rotavirus gastroenteritis|
|Number of pages||4|
|Journal||Giornale di Malattie Infettive e Parassitarie|
|Publication status||Published - 1984|
ASJC Scopus subject areas