TY - JOUR
T1 - Viral growth assay to evaluate the replicative capacity of HIV-1 isolates
AU - Nicastri, Emanuele
AU - Sarmati, Loredana
AU - Dori, Luca
AU - Montano, Marco
AU - D'Ettorre, Gabriella
AU - Buonomini, Anna Rita
AU - Parisi, Saverio G.
AU - Concia, Ercole
AU - Vullo, Vincenzo
AU - Andreoni, Massimo
PY - 2004/2
Y1 - 2004/2
N2 - The replicative capacity of HIV is studied by carrying out replication-competition experiments with the insertion of the gene of interest. These assays cannot capture the complicated patterns of mutations of different genes. A cross sectional study was carried out on 10 HIV-infected naïve patients and on 15 patients failing HAART. The CD8-depleted PBMCs, with known proviral DNA and cellular HIV-RNA copy numbers, were cultured. A reference curve was determined using the data obtained from 10 naïve patients. The replicative capacity was calculated as the ratio multiplied by 100 of the p24 antigen level of isolates over the p24 antigen level determined on the reference curve. A linear correlation between p24 antigen level and the infectious doses of HIV-DNA alone or plus cellular RNA copy number of PBMCs was found in naive patients (r=0.63, P
AB - The replicative capacity of HIV is studied by carrying out replication-competition experiments with the insertion of the gene of interest. These assays cannot capture the complicated patterns of mutations of different genes. A cross sectional study was carried out on 10 HIV-infected naïve patients and on 15 patients failing HAART. The CD8-depleted PBMCs, with known proviral DNA and cellular HIV-RNA copy numbers, were cultured. A reference curve was determined using the data obtained from 10 naïve patients. The replicative capacity was calculated as the ratio multiplied by 100 of the p24 antigen level of isolates over the p24 antigen level determined on the reference curve. A linear correlation between p24 antigen level and the infectious doses of HIV-DNA alone or plus cellular RNA copy number of PBMCs was found in naive patients (r=0.63, P
KW - Drug resistance
KW - HIV
KW - Replicative capacity
KW - Viral growth assay
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U2 - 10.1016/j.jviromet.2003.09.032
DO - 10.1016/j.jviromet.2003.09.032
M3 - Article
C2 - 14667536
AN - SCOPUS:10744219720
VL - 115
SP - 199
EP - 205
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 2
ER -