Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma

Elizabeth E. Brown; Denise Whitby; Francesco Vitale; Vickie Marshall; Georgina Mbisa; Christine Gamache; Carmela Lauria; Anthony J. Alberg; Diego Serraino; Paola Cordiali-Fei; Angelo Messina; James J. Goedert

doi:10.1002/cncr.22236

Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma

Elizabeth E. Brown, Denise Whitby, Francesco Vitale, Vickie Marshall, Georgina Mbisa, Christine Gamache, Carmela Lauria, Anthony J. Alberg, Diego Serraino, Paola Cordiali-Fei, Angelo Messina, James J. Goedert

Research output: Contribution to journal › Article

Abstract

BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (

Original language	English
Pages (from-to)	2282-2290
Number of pages	9
Journal	Cancer
Volume	107
Issue number	9
DOIs	https://doi.org/10.1002/cncr.22236
Publication status	Published - Nov 1 2006

Keywords

Biomarker
Human herpesvirus-8
Immunity
Kaposi sarcoma
Kaposi sarcoma-associated herpesvirus

ASJC Scopus subject areas

Cancer Research
Oncology

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Brown, E. E., Whitby, D., Vitale, F., Marshall, V., Mbisa, G., Gamache, C., Lauria, C., Alberg, A. J., Serraino, D., Cordiali-Fei, P., Messina, A., & Goedert, J. J. (2006). Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. Cancer, 107(9), 2282-2290. https://doi.org/10.1002/cncr.22236

Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. / Brown, Elizabeth E.; Whitby, Denise; Vitale, Francesco; Marshall, Vickie; Mbisa, Georgina; Gamache, Christine; Lauria, Carmela; Alberg, Anthony J.; Serraino, Diego; Cordiali-Fei, Paola; Messina, Angelo; Goedert, James J.

In: Cancer, Vol. 107, No. 9, 01.11.2006, p. 2282-2290.

Research output: Contribution to journal › Article

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abstract = "BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (",

keywords = "Biomarker, Human herpesvirus-8, Immunity, Kaposi sarcoma, Kaposi sarcoma-associated herpesvirus",

author = "Brown, {Elizabeth E.} and Denise Whitby and Francesco Vitale and Vickie Marshall and Georgina Mbisa and Christine Gamache and Carmela Lauria and Alberg, {Anthony J.} and Diego Serraino and Paola Cordiali-Fei and Angelo Messina and Goedert, {James J.}",

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AU - Brown, Elizabeth E.

AU - Whitby, Denise

AU - Vitale, Francesco

AU - Marshall, Vickie

AU - Mbisa, Georgina

AU - Gamache, Christine

AU - Lauria, Carmela

AU - Alberg, Anthony J.

AU - Serraino, Diego

AU - Cordiali-Fei, Paola

AU - Messina, Angelo

AU - Goedert, James J.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (

AB - BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (

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KW - Kaposi sarcoma

KW - Kaposi sarcoma-associated herpesvirus

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