Virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin E2

J. R. Panuska, F. Midulla, N. M. Cirino, A. Villani, I. A. Gilbert, E. R. McFadden, Y. T. Huang

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The cellular immune response to respiratory syncytial virus (RSV) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. Both tumor necrosis factor (TNF) and prostaglandin E2 (PGE2) are important regulatory mediators of the cellular immune response. We examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (MP) after RSV infection in vitro and compared production induced by virus with that induced by lipopolysaccharide (LPS). RSV infection of alveolar MP did not alter PGE2 production but increased expression of TNFα mRNA paralleled by increased secretion of immunoreactive and biologically active TNF. TNF production by alveolar MP was dependent on the infectious dose of virus and occurred early in the viral replication cycle. In contrast, RSV had minimal effects on blood MP production of TNF and PGE2. However, blood MP (and not alveolar MP) infected with RSV and costimulated with LPS demonstrated a 1.7-fold increase in PGE2 levels compared with LPS alone (P <0.001). Therefore, RSV has differential effects on human alveolar and blood MP production of these immunoregulatory molecules.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume259
Issue number6 3-3
Publication statusPublished - 1990

Fingerprint

Phagocytes
Dinoprostone
Tumor Necrosis Factor-alpha
Macrophages
Viruses
Respiratory Syncytial Viruses
Lipopolysaccharides
Respiratory Syncytial Virus Infections
Cellular Immunity
Virus Diseases
Pneumonia
Messenger RNA

Keywords

  • alveolar macrophage
  • cytokines
  • lipopolysaccharide

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin E2 . / Panuska, J. R.; Midulla, F.; Cirino, N. M.; Villani, A.; Gilbert, I. A.; McFadden, E. R.; Huang, Y. T.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 259, No. 6 3-3, 1990.

Research output: Contribution to journalArticle

Panuska, J. R. ; Midulla, F. ; Cirino, N. M. ; Villani, A. ; Gilbert, I. A. ; McFadden, E. R. ; Huang, Y. T. / Virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin E2 In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1990 ; Vol. 259, No. 6 3-3.
@article{e1e2a85c73564e469b863f3bf5f8cb2d,
title = "Virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin E2",
abstract = "The cellular immune response to respiratory syncytial virus (RSV) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. Both tumor necrosis factor (TNF) and prostaglandin E2 (PGE2) are important regulatory mediators of the cellular immune response. We examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (MP) after RSV infection in vitro and compared production induced by virus with that induced by lipopolysaccharide (LPS). RSV infection of alveolar MP did not alter PGE2 production but increased expression of TNFα mRNA paralleled by increased secretion of immunoreactive and biologically active TNF. TNF production by alveolar MP was dependent on the infectious dose of virus and occurred early in the viral replication cycle. In contrast, RSV had minimal effects on blood MP production of TNF and PGE2. However, blood MP (and not alveolar MP) infected with RSV and costimulated with LPS demonstrated a 1.7-fold increase in PGE2 levels compared with LPS alone (P <0.001). Therefore, RSV has differential effects on human alveolar and blood MP production of these immunoregulatory molecules.",
keywords = "alveolar macrophage, cytokines, lipopolysaccharide",
author = "Panuska, {J. R.} and F. Midulla and Cirino, {N. M.} and A. Villani and Gilbert, {I. A.} and McFadden, {E. R.} and Huang, {Y. T.}",
year = "1990",
language = "English",
volume = "259",
journal = "American Journal of Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "6 3-3",

}

TY - JOUR

T1 - Virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin E2

AU - Panuska, J. R.

AU - Midulla, F.

AU - Cirino, N. M.

AU - Villani, A.

AU - Gilbert, I. A.

AU - McFadden, E. R.

AU - Huang, Y. T.

PY - 1990

Y1 - 1990

N2 - The cellular immune response to respiratory syncytial virus (RSV) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. Both tumor necrosis factor (TNF) and prostaglandin E2 (PGE2) are important regulatory mediators of the cellular immune response. We examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (MP) after RSV infection in vitro and compared production induced by virus with that induced by lipopolysaccharide (LPS). RSV infection of alveolar MP did not alter PGE2 production but increased expression of TNFα mRNA paralleled by increased secretion of immunoreactive and biologically active TNF. TNF production by alveolar MP was dependent on the infectious dose of virus and occurred early in the viral replication cycle. In contrast, RSV had minimal effects on blood MP production of TNF and PGE2. However, blood MP (and not alveolar MP) infected with RSV and costimulated with LPS demonstrated a 1.7-fold increase in PGE2 levels compared with LPS alone (P <0.001). Therefore, RSV has differential effects on human alveolar and blood MP production of these immunoregulatory molecules.

AB - The cellular immune response to respiratory syncytial virus (RSV) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. Both tumor necrosis factor (TNF) and prostaglandin E2 (PGE2) are important regulatory mediators of the cellular immune response. We examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (MP) after RSV infection in vitro and compared production induced by virus with that induced by lipopolysaccharide (LPS). RSV infection of alveolar MP did not alter PGE2 production but increased expression of TNFα mRNA paralleled by increased secretion of immunoreactive and biologically active TNF. TNF production by alveolar MP was dependent on the infectious dose of virus and occurred early in the viral replication cycle. In contrast, RSV had minimal effects on blood MP production of TNF and PGE2. However, blood MP (and not alveolar MP) infected with RSV and costimulated with LPS demonstrated a 1.7-fold increase in PGE2 levels compared with LPS alone (P <0.001). Therefore, RSV has differential effects on human alveolar and blood MP production of these immunoregulatory molecules.

KW - alveolar macrophage

KW - cytokines

KW - lipopolysaccharide

UR - http://www.scopus.com/inward/record.url?scp=0025610892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025610892&partnerID=8YFLogxK

M3 - Article

VL - 259

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6119

IS - 6 3-3

ER -