Virus-specific CD8+ lymphocytes share the same effector-memory phenotype but exhibit functional differences in acute hepatitis B and C

Simona Urbani, Carolina Boni, Gabriele Missale, Gianfranco Elia, Cristina Cavallo, Marco Massari, Giovanni Raimondo, Carlo Ferrari

Research output: Contribution to journalArticle

Abstract

Hepatitis B and hepatitis C viruses (HBV and HCV) are both noncytopathic and can cause acute and chronic infections of the liver. Although they share tropism for the same organ, development of chronic hepatitis is much more frequent following HCV infection, suggesting different mechanisms of viral persistence. In this study, we show that circulating HBV- and HCV-specific tetramer-positive CD8 cells during the acute phase of hepatitis B and C belong almost entirely to an effector-memory subset (CCR7- CD45RA-). Despite this phenotypic similarity, HBV- and HCV-specific CD8 cells show striking functional differences. HBV-specific tetramer-positive CD8 cells express high perforin content ex vivo, expand vigorously, and display efficient cytotoxic activity and gamma interferon (IFN-γ) production upon peptide stimulation. A comparable degree of functional efficiency is maintained after the resolution of hepatitis B. In contrast, HCV-specific CD8 cells in the acute phase of hepatitis C express significantly lower levels of perforin molecules ex vivo and show depressed CD8 function in terms of proliferation, lytic activity, and IFN-γ production, irrespective of the final outcome of the disease. This defect is transient, because HCV-specific CD8 cells can progressively improve their function in patients with self-limited hepatitis C, while the CD8 function remains persistently depressed in subjects with a chronic evolution.

Original languageEnglish
Pages (from-to)12423-12434
Number of pages12
JournalJournal of Virology
Volume76
Issue number24
DOIs
Publication statusPublished - Dec 2002

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hepatitis C
hepatitis B
Hepatitis C
Hepatitis B
lymphocytes
Lymphocytes
Viruses
Phenotype
phenotype
viruses
Perforin
cells
chronic hepatitis
tropisms
Tropism
Hepatitis C virus
interferons
Chronic Hepatitis
Infection
interferon-gamma

ASJC Scopus subject areas

  • Immunology

Cite this

Virus-specific CD8+ lymphocytes share the same effector-memory phenotype but exhibit functional differences in acute hepatitis B and C. / Urbani, Simona; Boni, Carolina; Missale, Gabriele; Elia, Gianfranco; Cavallo, Cristina; Massari, Marco; Raimondo, Giovanni; Ferrari, Carlo.

In: Journal of Virology, Vol. 76, No. 24, 12.2002, p. 12423-12434.

Research output: Contribution to journalArticle

Urbani, S, Boni, C, Missale, G, Elia, G, Cavallo, C, Massari, M, Raimondo, G & Ferrari, C 2002, 'Virus-specific CD8+ lymphocytes share the same effector-memory phenotype but exhibit functional differences in acute hepatitis B and C', Journal of Virology, vol. 76, no. 24, pp. 12423-12434. https://doi.org/10.1128/JVI.76.24.12423-12434.2002
Urbani, Simona ; Boni, Carolina ; Missale, Gabriele ; Elia, Gianfranco ; Cavallo, Cristina ; Massari, Marco ; Raimondo, Giovanni ; Ferrari, Carlo. / Virus-specific CD8+ lymphocytes share the same effector-memory phenotype but exhibit functional differences in acute hepatitis B and C. In: Journal of Virology. 2002 ; Vol. 76, No. 24. pp. 12423-12434.
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