Visual observation of avidin-biotin affinity by fluorescent G4.5 poly(amidoamine) dendrimer

Govindachetty Saravanan, Kenji Daigo, Toyoko Imae, Takao Hamakubo

Research output: Contribution to journalArticle

Abstract

An air-treated G4.5 poly(amidoamine) (PAMAM) dendrimer displayed the enhanced fluorescence enough to be utilized as a fluorescence marker to visualize avidin-biotin affinity: On a fluorescence microscopic image, the avidin labeled by a fluorescent G4.5 PAMAM dendrimer was observed to be selectively bound on the biotin pattern that was prepared by amide-bonding of biotin on a carboxylic acid-terminated self-assembled monolayer and in turn by UV-irradiation with a photomask on the monolayer.

Original languageEnglish
Pages (from-to)58-60
Number of pages3
JournalColloids and Surfaces B: Biointerfaces
Volume83
Issue number1
DOIs
Publication statusPublished - Mar 1 2011

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Keywords

  • Avidin-biotin affinity
  • Fluorescence microscopy
  • Fluorescent dendrimer
  • Poly(amidoamine) dendrimer

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

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