Visualization of endocannabinoids in the cell

Sergio Oddi, Antonio Totaro, Mauro Maccarrone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A still unsolved, although critical, issue in endocannabinoid research is the mechanism by which the lipophilic compound anandamide (AEA) moves from its site of synthesis, crosses the aqueous milieu, and reaches the different intracellular compartments, where its metabolic and signaling pathways take place. The difficulty of studying intracellular AEA transport and distribution results from the lack of specific probes and techniques to track and visualize this bioactive lipid within the cell. Here, we describe the use of a biotinylated, non-hydrolyzable derivative of AEA (biotin-AEA, b-AEA) for visualizing the subcellular distribution of this endocannabinoid by means of confocal fluorescence microscopy.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-282
Number of pages6
Volume1412
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)10643745

Keywords

  • Anandamide
  • Biotinylated derivatives
  • Confocal microscopy
  • Endocannabinoids
  • Glass cover slips
  • Subcellular distribution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Visualization of endocannabinoids in the cell'. Together they form a unique fingerprint.

Cite this