TY - JOUR
T1 - Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy
AU - Mironov, Alexander A.
AU - Beznoussenko, Galina V.
AU - Luini, Alberto
AU - Polishchuk, Roman S.
PY - 2006
Y1 - 2006
N2 - The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. This approach is based on such an association of two techniques. An individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and a "snapshot" of its three-dimensional (3-D) ultrastructure and especially tomographic reconstruction can then be taken at any chosen time during its life cycle. The pitfalls and potential of this approach are discussed.
AB - The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. This approach is based on such an association of two techniques. An individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and a "snapshot" of its three-dimensional (3-D) ultrastructure and especially tomographic reconstruction can then be taken at any chosen time during its life cycle. The pitfalls and potential of this approach are discussed.
UR - http://www.scopus.com/inward/record.url?scp=30544436712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30544436712&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(05)04005-X
DO - 10.1016/S0076-6879(05)04005-X
M3 - Article
C2 - 16413256
VL - 404
SP - 43
EP - 57
JO - ImmunoMethods
JF - ImmunoMethods
SN - 1046-2023
M1 - 5
ER -