Vitamin D: An instrumental factor in the anti-phospholipid syndrome by inhibition of tissue factor expression

N. Agmon-Levin, M. Blank, G. Zandman-Goddard, H. Orbach, P. L. Meroni, A. Tincani, A. Doria, R. Cervera, W. Miesbach, L. Stojanovich, V. Barak, B. S. Porat-Katz, H. Amital, Y. Shoenfeld

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aims Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterised by thrombosis, obstetric complications and the presence of anti-phospholipid antibodies such as anti-β2GPI-Abs. These antibodies may set off the coagulation cascade via several mechanisms, including the induction of tissue factor (TF) expression. Vitamin D has recently emerged as an immunomodulator that might exert an anti-thrombotic effect. Therefore, we studied serum vitamin D levels in a cohort of APS patients, as well as the effect of vitamin D in an in vitro model of APS-mediated thrombosis. Methods: Serum vitamin D levels were measured in 179 European APS patients and 141 healthy controls using the LIAISON chemiluminescent immunoassay, and the levels were evaluated in conjunction with a wide spectrum of clinical manifestations. In an vitro model, anti-β2GPI antibodies were purified from four patients with APS to evaluate the expression of TF in activated starved human umbilical vein endothelial cells. The effect of vitamin D (1,25-dihydroxyvitamin D, 10 nm) on anti-β2GPI-Abs mediated TF expression was analysed by immunoblot. Results: Vitamin D deficiency (serum level ≤15 ng/ml) was documented in 49.5% of our APS patients versus 30% of controls (p

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalAnnals of the Rheumatic Diseases
Volume70
Issue number1
DOIs
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Allergy

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