Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)

Cristina O'Callaghan-Gordo, Manolis Kogevinas, Eleni Fthenou, Marie Pedersen, Ana Espinosa, Georgia Chalkiadaki, Vasiliki Daraki, Eirini Dermitzaki, Ilse Decordier, Vaggelis Georgiou, Domenico Franco Merlo, Theano Roumeliotaki, Kim Vande Loock, Jos Kleinjans, Micheline Kirsch-Volders, Leda Chatzi

Research output: Contribution to journalArticle

Abstract

Background & aims: Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods: 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results: Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion: Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

Original languageEnglish
JournalClinical Nutrition
DOIs
Publication statusAccepted/In press - Jan 18 2016

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Rheiformes
Greece
Vitamin D
Mothers
Newborn Infant
T-Lymphocytes
Pregnancy
Fetal Blood
Vitamin D Deficiency
Food
Micronucleus Tests
Cytokinesis
Second Pregnancy Trimester
First Pregnancy Trimester
Serum
Birth Weight
DNA Damage
Pregnant Women
Neoplasms
Biomarkers

Keywords

  • Childhood cancer
  • Genotoxicity
  • In utero exposures
  • Micronuclei
  • Mother-child cohort
  • Vitamin D

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Critical Care and Intensive Care Medicine

Cite this

Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete). / O'Callaghan-Gordo, Cristina; Kogevinas, Manolis; Fthenou, Eleni; Pedersen, Marie; Espinosa, Ana; Chalkiadaki, Georgia; Daraki, Vasiliki; Dermitzaki, Eirini; Decordier, Ilse; Georgiou, Vaggelis; Merlo, Domenico Franco; Roumeliotaki, Theano; Vande Loock, Kim; Kleinjans, Jos; Kirsch-Volders, Micheline; Chatzi, Leda.

In: Clinical Nutrition, 18.01.2016.

Research output: Contribution to journalArticle

O'Callaghan-Gordo, C, Kogevinas, M, Fthenou, E, Pedersen, M, Espinosa, A, Chalkiadaki, G, Daraki, V, Dermitzaki, E, Decordier, I, Georgiou, V, Merlo, DF, Roumeliotaki, T, Vande Loock, K, Kleinjans, J, Kirsch-Volders, M & Chatzi, L 2016, 'Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete)', Clinical Nutrition. https://doi.org/10.1016/j.clnu.2016.06.016
O'Callaghan-Gordo, Cristina ; Kogevinas, Manolis ; Fthenou, Eleni ; Pedersen, Marie ; Espinosa, Ana ; Chalkiadaki, Georgia ; Daraki, Vasiliki ; Dermitzaki, Eirini ; Decordier, Ilse ; Georgiou, Vaggelis ; Merlo, Domenico Franco ; Roumeliotaki, Theano ; Vande Loock, Kim ; Kleinjans, Jos ; Kirsch-Volders, Micheline ; Chatzi, Leda. / Vitamin D insufficient levels during pregnancy and micronuclei frequency in peripheral blood T lymphocytes mothers and newborns (Rhea cohort, Crete). In: Clinical Nutrition. 2016.
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abstract = "Background & aims: Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods: 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results: Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95{\%}CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion: Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.",
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AU - O'Callaghan-Gordo, Cristina

AU - Kogevinas, Manolis

AU - Fthenou, Eleni

AU - Pedersen, Marie

AU - Espinosa, Ana

AU - Chalkiadaki, Georgia

AU - Daraki, Vasiliki

AU - Dermitzaki, Eirini

AU - Decordier, Ilse

AU - Georgiou, Vaggelis

AU - Merlo, Domenico Franco

AU - Roumeliotaki, Theano

AU - Vande Loock, Kim

AU - Kleinjans, Jos

AU - Kirsch-Volders, Micheline

AU - Chatzi, Leda

PY - 2016/1/18

Y1 - 2016/1/18

N2 - Background & aims: Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods: 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results: Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion: Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

AB - Background & aims: Vitamin D deficiency is common among pregnant women and may be associated with several adverse health outcomes including cancer. Micronuclei frequency is a biomarker of early genetic effects and has been used to examine the association between genotoxic exposures and cancer. We examined maternal vitamin D levels during pregnancy in associations with micronuclei frequency in maternal blood and in cord blood. Methods: 173 mothers and 171 newborns born between 2007 and 2008 in Heraklion (Crete, Greece) were included in the study. Between 14th and 18th weeks of gestation we collected information on maternal diet using food frequency questionnaires (FFQs). We measured maternal serum concentrations of 25-hydroxyvitamin D [25(OH)D] between the first and second trimester of pregnancy. We estimated dietary vitamin D intake using information from FFQ. After delivery we collected cord blood and maternal peripheral blood. We used the cytokinesis-block micronucleus (CBMN) assay to assess the frequencies of micronucleated cells in binucleated T lymphocytes (MNBN). Results: Maternal insufficient serum levels of 25(OH)D (<50 nmol/L) during pregnancy were associated with increased MNBN frequency in cord blood [IRR = 1.32 (95%CI: 1.00, 1.72)]. This increase was higher for newborns with birth weight above the third quartile [≥3.500 kg; IRR = 2.21 (1.26, 3.89)]. Similarly, low levels of dietary vitamin D were associated with increased MNBN frequency in cord blood [middle tertile IRR = 1.08 (0.78, 1.47), lower tertile IRR = 1.51 (1.06, 2.14)]. Insufficient levels of vitamin D were not associated with MNBN in mothers. Conclusion: Our results suggest that vitamin D deficiency during pregnancy increases genotoxic risks in newborns. The prevalence of vitamin D deficiency globally is high and it is important to further investigate whether vitamin D supplementation or similar interventions during pregnancy could prevent DNA damage at early stages of life.

KW - Childhood cancer

KW - Genotoxicity

KW - In utero exposures

KW - Micronuclei

KW - Mother-child cohort

KW - Vitamin D

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