Abstract
This study was designed to evaluate the time-dependent changes of mitochondrial membrane potential and mass during Con-A-induced proliferation of splenic lymphocytes from rat fed a normal or a vitamin E deficient diet. Rhodamine 123 and Nonyl Acridine Orange were used as specific probes to monitor the membrane potential and mass of mitochondria, respectively, by means of flow cytometry. The results demonstrate that the increase of Rh-123 and NAO uptake observed in cells from normally fed rats was prevented by vitamin E deficiency, at any time considered. After 72 h from Con A stimulation, 62% of cells from controls, as against 16% of cells from vitamin E deficient rats, showed hyperpolarized mitochondria. At the same time, in this last group, 60% of cells had depolarized organelles. The same pattern was observed considering the changes of mitochondrial mass, measured using NAO as a probe. These data support that mitogenic stimulation induced an increase of the respiratory activity of mitochondria with subsequent production of superoxide radicals. This resulted in depolarization and loss of mass of the organelles if the intracellular level of vitamin E is not adequate.
| Original language | English |
|---|---|
| Pages (from-to) | 661-665 |
| Number of pages | 5 |
| Journal | Free Radical Biology and Medicine |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- Free radicals
- Lymphocyte proliferation
- Membrane potential
- Mitochondria
- Mitochondrial mass
- Vitamin E
ASJC Scopus subject areas
- Clinical Biochemistry
- Medicine(all)
- Toxicology
Cite this
Vitamin E deficiency impairs the modifications of mitochondrial membrane potential and mass in rat splenocytes stimulated to proliferate. / Pieri, Carlo; Moroni, Fausto; Recchioni, Rina.
In: Free Radical Biology and Medicine, Vol. 15, No. 6, 1993, p. 661-665.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vitamin E deficiency impairs the modifications of mitochondrial membrane potential and mass in rat splenocytes stimulated to proliferate
AU - Pieri, Carlo
AU - Moroni, Fausto
AU - Recchioni, Rina
PY - 1993
Y1 - 1993
N2 - This study was designed to evaluate the time-dependent changes of mitochondrial membrane potential and mass during Con-A-induced proliferation of splenic lymphocytes from rat fed a normal or a vitamin E deficient diet. Rhodamine 123 and Nonyl Acridine Orange were used as specific probes to monitor the membrane potential and mass of mitochondria, respectively, by means of flow cytometry. The results demonstrate that the increase of Rh-123 and NAO uptake observed in cells from normally fed rats was prevented by vitamin E deficiency, at any time considered. After 72 h from Con A stimulation, 62% of cells from controls, as against 16% of cells from vitamin E deficient rats, showed hyperpolarized mitochondria. At the same time, in this last group, 60% of cells had depolarized organelles. The same pattern was observed considering the changes of mitochondrial mass, measured using NAO as a probe. These data support that mitogenic stimulation induced an increase of the respiratory activity of mitochondria with subsequent production of superoxide radicals. This resulted in depolarization and loss of mass of the organelles if the intracellular level of vitamin E is not adequate.
AB - This study was designed to evaluate the time-dependent changes of mitochondrial membrane potential and mass during Con-A-induced proliferation of splenic lymphocytes from rat fed a normal or a vitamin E deficient diet. Rhodamine 123 and Nonyl Acridine Orange were used as specific probes to monitor the membrane potential and mass of mitochondria, respectively, by means of flow cytometry. The results demonstrate that the increase of Rh-123 and NAO uptake observed in cells from normally fed rats was prevented by vitamin E deficiency, at any time considered. After 72 h from Con A stimulation, 62% of cells from controls, as against 16% of cells from vitamin E deficient rats, showed hyperpolarized mitochondria. At the same time, in this last group, 60% of cells had depolarized organelles. The same pattern was observed considering the changes of mitochondrial mass, measured using NAO as a probe. These data support that mitogenic stimulation induced an increase of the respiratory activity of mitochondria with subsequent production of superoxide radicals. This resulted in depolarization and loss of mass of the organelles if the intracellular level of vitamin E is not adequate.
KW - Free radicals
KW - Lymphocyte proliferation
KW - Membrane potential
KW - Mitochondria
KW - Mitochondrial mass
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=0027514831&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027514831&partnerID=8YFLogxK
U2 - 10.1016/0891-5849(93)90170-Y
DO - 10.1016/0891-5849(93)90170-Y
M3 - Article
C2 - 7511124
AN - SCOPUS:0027514831
VL - 15
SP - 661
EP - 665
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 6
ER -