Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus

Mauro Provinciali, Oscar Cirioni, Fiorenza Orlando, Elisa Pierpaoli, Alessandra Barucca, Carmela Silvestri, Roberto Ghiselli, Alessandro Scalise, Lucia Brescini, Mario Guerrieri, Andrea Giacometti

Research output: Contribution to journalArticlepeer-review

Abstract

A relevant bacterial load in cutaneous wounds significantly interferes with the normal process of healing. Vitamin E (VE) is a known immunomodulator and immune enhancer. Here, it was shown that administration of VE before infection was effective at increasing the antimicrobial activity of daptomycin (DAP) or tigecycline (TIG) in a mouse model of wound infection caused by meticillinresistant Staphylococcus aureus (MRSA). A wound was established through the panniculus carnosus of mice and inoculated with MRSA. Mice were assigned to six groups: a VE pre-treated group with no antibiotics given after MRSA challenge; two VE pre-treated groups with DAP or TIG given after MRSA challenge; two groups treated with DAP or TIG only after MRSA challenge; and a control group that did not receive any treatment. Mice receiving each antibiotic alone showed a 3 log decrease in the number of c.f.u. recovered compared with the control group, mice treated with VE plus TIG had a 4 log decrease, whilst mice treated with VE plus DAP had the largest decrease in c.f.u. recovered (5 logs). The increased antimicrobial effect seen from treatment with VE plus antibiotics was associated with increased levels of natural killer cell cytotoxicity, with a more pronounced increase in leukocyte populations in mice treated with VE plus DAP. These data suggest that treatment with VE prior to infection and subsequent antibiotic treatment act in synergy.

Original languageEnglish
Pages (from-to)1806-1812
Number of pages7
JournalJournal of Medical Microbiology
Volume60
Issue number12
DOIs
Publication statusPublished - Dec 2011

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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