Vitamin E suppresses 5-lipoxygenase-mediated oxidative stress in peripheral blood mononuclear cells of hemodialysis patients regardless of administration route

Mauro Maccarrone, Carlo Meloni, Simone Manca-di-Villahermosa, Nella Cococcetta, Carlo U. Casciani, Alessandro Finazzi-Agrò, Massimo Taccone-Gallucci

Research output: Contribution to journalArticle

Abstract

A number of pathological conditions caused by oxidative stress have been reported in uremic patients undergoing maintenance hemodialysis (HD). Enhanced lipid peroxidation was previously observed in peripheral blood mononuclear cells (PBMCs) of HD patients. Upregulation of 5-lipoxygenase (5-Lox) activity and protein content with enhanced production of leukotriene B4(LTB4) and membrane lipoperoxides was also shown in PBMCs of HD patients. Administration of free vitamin E specifically inhibited 5-Lox activity without affecting gene expression at the protein level. To assess whether oral or intramuscular (IM) administration of vitamin E may suppress 5-Lox in HD patients, PBMCs from 16 subjects on maintenance HD therapy for at least 6 months were investigated before and after a short course of IM or oral administration of vitamin E (8 patients per group). PBMCs from 13 healthy controls were also evaluated and assumed as the reference standard. Vitamin E significantly reduced lipid peroxidation, LTB4 content, and 5-Lox activity in PBMCs, whereas 5-Lox gene expression at the protein level was not affected. There were no significant differences in these parameters between patients treated with IM or oral vitamin E. PBMCs of HD patients showed enhanced membrane lipid peroxidation and release of LTB4, both linked to upregulation of 5-Lox. 5-Lox activity and related oxidative stress were significantly (although not completely) suppressed by vitamin E regardless of the administration route.

Original languageEnglish
Pages (from-to)964-969
Number of pages6
JournalAmerican Journal of Kidney Diseases
Volume37
Issue number5
Publication statusPublished - 2001

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Keywords

  • 5-Lipoxygenase (5-Lox)
  • Hemodialysis (HD)
  • Lipid peroxidation
  • Oxidative stress
  • Peripheral blood mononuclear cells (PBMCs)
  • Vitamin E administration

ASJC Scopus subject areas

  • Nephrology

Cite this

Maccarrone, M., Meloni, C., Manca-di-Villahermosa, S., Cococcetta, N., Casciani, C. U., Finazzi-Agrò, A., & Taccone-Gallucci, M. (2001). Vitamin E suppresses 5-lipoxygenase-mediated oxidative stress in peripheral blood mononuclear cells of hemodialysis patients regardless of administration route. American Journal of Kidney Diseases, 37(5), 964-969.