Vitamin K (VK) compounds have previously been found to inhibit hepatoma cell line growth. In this study, we investigated their activity on normal rat hepatocyte DNA synthesis (DNA-S) in vitro and in vivo using VK2 (menaquinone) and compound 5 (a thio-alkyl analog of VK3)-JBC 1995. 270. 28304. Both VK2 and Cpd 5 (675 and 20microM) inhibited EGF-induced DNA-S (>90%) in primary hepatocyte cultures in vitro. VK2 and Cpd 5 (675 and 120 micromole/kg) also inhibited liver DNA-S at 24 hr. post 2/3 partial hepatectomy (PH) in vivo. At 24 hr. post PH, TGF6 mRNA expression was suppressed but c-jun mRNA was induced in VK treated rats compared with controls. There were no differences in the serum levels of SGPT, GGTP or albumin between treated or control rats. Results indicate that VK2 and Cpd 5 inhibit normal hepatocyte DNA-S in the GI phase of the cell cycle, in vitro and in vivo without measurable hepatotoxicity.
|Number of pages||1|
|Publication status||Published - 1997|
ASJC Scopus subject areas
- Cancer Research
- Cell Biology