Voltage gating of Ca2+-activated potassium channels in human lymphocytes

László Mátyus, Carlo Pieri, Rina Recchioni, Fausto Moroni, László Bene, Lajos Trón, Sándor Damjanovich

Research output: Contribution to journal › Article

Abstract

The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number1
DOIs
Publication statusPublished - Aug 31 1990

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Calcium-Activated Potassium Channels
Lymphocytes
Potassium Channels
Membrane Potentials
Potassium Channel Blockers
Membranes
Ionomycin
Flow cytometry
Depolarization
Electric potential
Flow Cytometry
Cells
Population

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Voltage gating of Ca2+-activated potassium channels in human lymphocytes. / Mátyus, László; Pieri, Carlo; Recchioni, Rina; Moroni, Fausto; Bene, László; Trón, Lajos; Damjanovich, Sándor.

In: Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 31.08.1990, p. 325-329.

Research output: Contribution to journal › Article

Mátyus, L, Pieri, C, Recchioni, R, Moroni, F, Bene, L, Trón, L & Damjanovich, S 1990, 'Voltage gating of Ca2+-activated potassium channels in human lymphocytes', Biochemical and Biophysical Research Communications, vol. 171, no. 1, pp. 325-329. https://doi.org/10.1016/0006-291X(90)91396-A
Mátyus L, Pieri C, Recchioni R, Moroni F, Bene L, Trón L et al. Voltage gating of Ca2+-activated potassium channels in human lymphocytes. Biochemical and Biophysical Research Communications. 1990 Aug 31;171(1):325-329. https://doi.org/10.1016/0006-291X(90)91396-A
Mátyus, László ; Pieri, Carlo ; Recchioni, Rina ; Moroni, Fausto ; Bene, László ; Trón, Lajos ; Damjanovich, Sándor. / Voltage gating of Ca2+-activated potassium channels in human lymphocytes. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 171, No. 1. pp. 325-329.
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