Voltage gating of Ca2+-activated potassium channels in human lymphocytes

László Mátyus; Carlo Pieri; Rina Recchioni; Fausto Moroni; László Bene; Lajos Trón; Sándor Damjanovich

doi:10.1016/0006-291X(90)91396-A

Voltage gating of Ca²⁺-activated potassium channels in human lymphocytes

László Mátyus, Carlo Pieri, Rina Recchioni, Fausto Moroni, László Bene, Lajos Trón, Sándor Damjanovich

Istituto Nazionale di Riposo e Cura per Anziani V.E. II

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of membrane potential on Ca²⁺ activated K⁺ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca²⁺ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca²⁺ caused hyperpolarization. However 1 mM Ca²⁺ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca²⁺ activated K⁺ channels is suggested.

Original language	English
Pages (from-to)	325-329
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	171
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91396-A
Publication status	Published - Aug 31 1990

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Voltage gating of Ca2+-activated potassium channels in human lymphocytes",

abstract = "The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.",

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AU - Mátyus, László

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AU - Recchioni, Rina

AU - Moroni, Fausto

AU - Bene, László

AU - Trón, Lajos

AU - Damjanovich, Sándor

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