Voltage gating of Ca2+-activated potassium channels in human lymphocytes

László Mátyus, Carlo Pieri, Rina Recchioni, Fausto Moroni, László Bene, Lajos Trón, Sándor Damjanovich

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number1
DOIs
Publication statusPublished - Aug 31 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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