TY - JOUR
T1 - Voltage gating of Ca2+-activated potassium channels in human lymphocytes
AU - Mátyus, László
AU - Pieri, Carlo
AU - Recchioni, Rina
AU - Moroni, Fausto
AU - Bene, László
AU - Trón, Lajos
AU - Damjanovich, Sándor
PY - 1990/8/31
Y1 - 1990/8/31
N2 - The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.
AB - The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 μM ionomycin depolarized the control cell population, while 100 μM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.
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U2 - 10.1016/0006-291X(90)91396-A
DO - 10.1016/0006-291X(90)91396-A
M3 - Article
C2 - 2393396
AN - SCOPUS:0025193232
VL - 171
SP - 325
EP - 329
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -