Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes

Davide Ravelli, Paola Isernia, Andrea Acquarulo, Antonella Profumo, Daniele Merli

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin (HSA)) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin-drug interaction parameters, including the replacement of the drug-albumin adduct by a competitive compound, sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.

Original languageEnglish
Pages (from-to)10110-10115
Number of pages6
JournalAnalytical Chemistry
Volume91
Issue number15
DOIs
Publication statusPublished - Aug 6 2019

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Stoichiometry
Albumins
Pharmaceutical Preparations
Drug interactions
Pharmacokinetics
Glassy carbon
Voltammetry
Complexation
Serum Albumin
Electrolytes
Availability
Electrodes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes. / Ravelli, Davide; Isernia, Paola; Acquarulo, Andrea; Profumo, Antonella; Merli, Daniele.

In: Analytical Chemistry, Vol. 91, No. 15, 06.08.2019, p. 10110-10115.

Research output: Contribution to journalArticle

Ravelli, Davide ; Isernia, Paola ; Acquarulo, Andrea ; Profumo, Antonella ; Merli, Daniele. / Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes. In: Analytical Chemistry. 2019 ; Vol. 91, No. 15. pp. 10110-10115.
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