Abstract
The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin (HSA)) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin-drug interaction parameters, including the replacement of the drug-albumin adduct by a competitive compound, sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.
| Original language | English |
|---|---|
| Pages (from-to) | 10110-10115 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 91 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Aug 6 2019 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes. / Ravelli, Davide; Isernia, Paola; Acquarulo, Andrea; Profumo, Antonella; Merli, Daniele.
In: Analytical Chemistry, Vol. 91, No. 15, 06.08.2019, p. 10110-10115.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes
AU - Ravelli, Davide
AU - Isernia, Paola
AU - Acquarulo, Andrea
AU - Profumo, Antonella
AU - Merli, Daniele
PY - 2019/8/6
Y1 - 2019/8/6
N2 - The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin (HSA)) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin-drug interaction parameters, including the replacement of the drug-albumin adduct by a competitive compound, sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.
AB - The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin (HSA)) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin-drug interaction parameters, including the replacement of the drug-albumin adduct by a competitive compound, sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.
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U2 - 10.1021/acs.analchem.9b02088
DO - 10.1021/acs.analchem.9b02088
M3 - Article
VL - 91
SP - 10110
EP - 10115
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 15
ER -