Voltammetric determination of binding constant and stoichiometry of albumin (Human, Bovine, Ovine)-Drug Complexes

Davide Ravelli, Paola Isernia, Andrea Acquarulo, Antonella Profumo, Daniele Merli

Research output: Contribution to journalArticle

Abstract

The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin (HSA)) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin-drug interaction parameters, including the replacement of the drug-albumin adduct by a competitive compound, sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.

Original languageEnglish
Pages (from-to)10110-10115
Number of pages6
JournalAnalytical Chemistry
Volume91
Issue number15
DOIs
Publication statusPublished - Aug 6 2019

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ASJC Scopus subject areas

  • Analytical Chemistry

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