Volume-regulated Cl- channels in human pleural mesothelioma cells

Giuliano Meyer, Simona Rodighiero, Fabiana Guizzardi, Claudia Bazzini, Guido Bottà, Cristina Bertocchi, Lisa Garavaglia, Silvia Dossena, Rosangela Manfredi, Chiara Sironi, Anna Catania, Markus Paulmichl

Research output: Contribution to journalArticlepeer-review


Anion channels in human mesothelial and mesothelioma cell lines were characterized by patch-clamp and biomolecular approaches. We found an outwardly rectifying anionic current which was inactivated at positive voltages and inhibited by extracellular adenosine 5′-triphosphate (ATP). Mesothelial and mesothelioma cells behaved differently concerning current inactivation properties. Inactivation is more pronounced and has a steeper onset in mesothelial cells. Different reversal potentials, in asymmetrical Cl - solutions, that could be attributed to a different selectivity of the channel, have been observed in the two cell lines. Mesothelioma cell single-channel analysis indicates that the number of the same active anion channel (3-4 pS) increased under hypoosmotic conditions. Immunocytochemistry experiments showed the presence of ICln protein in the cytosol and in the plasma membrane. Western blot analysis revealed an increase of ICln in the membrane under hypotonic conditions, an event possibly related to the activation of Cl- channels.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalFEBS Letters
Issue number1-3
Publication statusPublished - Feb 13 2004


  • Adenosine 5′-triphosphate inhibition
  • Cl channel
  • ICln
  • Immunocytochemistry
  • Pleural mesothelium
  • Regulatory volume decrease

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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