Weak effect of neuromodulators on climbing fiber-activated [Ca2+](i) increases in rat cerebellar Purkinje neurons

Antonio Pisani, William N. Ross

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca2+](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca2+ indicator bis-fura-2 with patch electrodes on the soma. [Ca2+](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca2+](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalBrain Research
Volume831
Issue number1-2
DOIs
Publication statusPublished - Jun 12 1999

Keywords

  • Calcium
  • Climbing fiber
  • Dendrite
  • Neuromodulator
  • Purkinje cell

ASJC Scopus subject areas

  • Neuroscience(all)

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