Abstract
The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca2+](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca2+ indicator bis-fura-2 with patch electrodes on the soma. [Ca2+](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca2+](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.
Original language | English |
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Pages (from-to) | 113-118 |
Number of pages | 6 |
Journal | Brain Research |
Volume | 831 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Jun 12 1999 |
Keywords
- Calcium
- Climbing fiber
- Dendrite
- Neuromodulator
- Purkinje cell
ASJC Scopus subject areas
- Neuroscience(all)