Weak effect of neuromodulators on climbing fiber-activated [Ca2+](i) increases in rat cerebellar Purkinje neurons

Antonio Pisani; William N. Ross

doi:10.1016/S0006-8993(99)01406-7

Weak effect of neuromodulators on climbing fiber-activated [Ca2+](i) increases in rat cerebellar Purkinje neurons

Antonio Pisani, William N. Ross

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca²⁺](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca²⁺ indicator bis-fura-2 with patch electrodes on the soma. [Ca²⁺](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca²⁺](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.

Original language	English
Pages (from-to)	113-118
Number of pages	6
Journal	Brain Research
Volume	831
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(99)01406-7
Publication status	Published - Jun 12 1999

Keywords

Calcium
Climbing fiber
Dendrite
Neuromodulator
Purkinje cell

ASJC Scopus subject areas

Neuroscience(all)

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title = "Weak effect of neuromodulators on climbing fiber-activated [Ca2+](i) increases in rat cerebellar Purkinje neurons",

abstract = "The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca2+](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca2+ indicator bis-fura-2 with patch electrodes on the soma. [Ca2+](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca2+](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.",

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AU - Ross, William N.

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N2 - The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca2+](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca2+ indicator bis-fura-2 with patch electrodes on the soma. [Ca2+](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca2+](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.

AB - The effect of several neuromodulators (carbachol (CCh), serotonin (5- HT), noradrenaline (NE), and dopamine (DA)) on the climbing fiber (CF)- induced [Ca2+](i) increase in the dendrites of cerebellar Purkinje cells was examined in slices from the rat cerebellum. Purkinje cells were filled with the Ca2+ indicator bis-fura-2 with patch electrodes on the soma. [Ca2+](i) changes were measured from regions of interest in the dendrites with a high speed camera. Changes evoked by one or three responses were measured in control conditions and with neuromodulators added to the bath. None of these four classic modulators caused a significant change in the CF- induced [Ca2+](i) amplitude. Buspirone, a partial 5-HT(1A) agonist and a weak DA receptor antagonist caused a small (10-15%) reduction in the response.

KW - Calcium

KW - Climbing fiber

KW - Dendrite

KW - Neuromodulator

KW - Purkinje cell

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ER -

Weak effect of neuromodulators on climbing fiber-activated [Ca2+](i) increases in rat cerebellar Purkinje neurons

Abstract

Keywords

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