Weak platelet agonists and U46619 induce apoptosis-like events in platelets, in the absence of phosphatidylserine exposure

Giovanni Tonon, Xunyi Luo, Nicholas J. Greco, Weidong Chen, Yufang Shi, Graham A. Jamieson

Research output: Contribution to journalArticle

Abstract

Platelets express apoptotic markers during storage, while aging and after stimulation with strong agonists thrombin and collagen. It is unknown if the weak agonists ADP and epinephrine or U46619, a thromboxane analog, induce the expression of apoptotic markers in platelets. To answer this question, we measured phosphatidylserine exposure, gelsolin cleavage and decrease in membrane mitochondrial potential after stimulation with these agonists. No phosphatidylserine exposure was evident, however, gelsolin cleavage and a platelet population with a decreased membrane mitochondrial potential appeared, suggesting that in platelets selective agonists can induce apoptosis in the absence of phosphatidylserine exposure. Interestingly, costimulation by thrombin plus collagen together with each of the other agonists increased the phosphatidylserine exposure induced by strong agonists. These findings may be of importance in platelet activation and apoptosis under pathophysiological conditions where multiple effectors are involved.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalThrombosis Research
Volume107
Issue number6
DOIs
Publication statusPublished - Sep 15 2002

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Keywords

  • Annexin V
  • Apoptosis
  • Phosphatidylserine
  • Platelet
  • Platelet transfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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