TY - JOUR
T1 - Weight loss reduces adipose tissue cathepsin S and its circulating levels in morbidly obese women
AU - Taleb, Soraya
AU - Cancello, Raffaella
AU - Poitou, Christine
AU - Rouault, Christine
AU - Sellam, Philippe
AU - Levy, Patrick
AU - Bouillot, Jean Luc
AU - Coussieu, Christiane
AU - Basdevant, Arnaud
AU - Guerre-Millo, Michèle
AU - Lacasa, Danièle
AU - Clement, Karine
PY - 2006/3
Y1 - 2006/3
N2 - Context: Human adipose tissue produces several adipokines, including the newly identified protein cathepsinS(CTSS), a cysteine protease involved in the pathogenesis of atherosclerosis. Obesity is characterized by high levels of CTSS in the circulation and in sc white adipose tissue (scWAT). Objective: We investigated the effect of surgery-induced weight loss on circulating CTSS and its protein expression in scWAT. Design: Fifty morbidly obese women before and 3 months after surgery and 10 healthy lean women were studied. We analyzed the relationships between circulating CTSS and clinical and biological parameters. Immunohistochemistry of the CTSS protein variations in scWAT was performed. Results: Weight loss decreased by 42% (P <0.0001) the circulating CTSS levels, which correlated with changes in body weight (P = 0.03). Weobserved a significant decrease in CTSS enzymatic activity by 25% after weight loss (P = 0.001). Adipose tissue CTSS content was reduced by 30% (P = 0.002) after surgery. The variations in CTSS expression in scWAT after surgery correlated with changes in circulating CTSS serum levels (P = 0.03). Most of the correlations between CTSS and clinical and biological parameters disappeared after adjustment for body mass index, emphasizing the strong link between CTSS and corpulence in humans. Conclusions: Changes in CTSS scWAT might contribute to serum variations in CTSS during weight loss. The decrease in CTSS concentrations in the circulation may contribute to vascular improvement in obese subjects after weight loss.
AB - Context: Human adipose tissue produces several adipokines, including the newly identified protein cathepsinS(CTSS), a cysteine protease involved in the pathogenesis of atherosclerosis. Obesity is characterized by high levels of CTSS in the circulation and in sc white adipose tissue (scWAT). Objective: We investigated the effect of surgery-induced weight loss on circulating CTSS and its protein expression in scWAT. Design: Fifty morbidly obese women before and 3 months after surgery and 10 healthy lean women were studied. We analyzed the relationships between circulating CTSS and clinical and biological parameters. Immunohistochemistry of the CTSS protein variations in scWAT was performed. Results: Weight loss decreased by 42% (P <0.0001) the circulating CTSS levels, which correlated with changes in body weight (P = 0.03). Weobserved a significant decrease in CTSS enzymatic activity by 25% after weight loss (P = 0.001). Adipose tissue CTSS content was reduced by 30% (P = 0.002) after surgery. The variations in CTSS expression in scWAT after surgery correlated with changes in circulating CTSS serum levels (P = 0.03). Most of the correlations between CTSS and clinical and biological parameters disappeared after adjustment for body mass index, emphasizing the strong link between CTSS and corpulence in humans. Conclusions: Changes in CTSS scWAT might contribute to serum variations in CTSS during weight loss. The decrease in CTSS concentrations in the circulation may contribute to vascular improvement in obese subjects after weight loss.
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U2 - 10.1210/jc.2005-1601
DO - 10.1210/jc.2005-1601
M3 - Article
C2 - 16394095
AN - SCOPUS:33644817971
VL - 91
SP - 1042
EP - 1047
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 3
ER -