Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects

Karine Clément, Nathalie Viguerie, Christine Poitou, Claire Carette, Véronique Pelloux, Cyrile A. Curat, Audrey Sicard, Sophie Rome, Arriel Benis, Jean Daniel Zucker, Hubert Vidal, Martine Laville, Gregory S. Barsh, Arnaud Basdevant, Vladimir Stich, Raffaella Cancello, Dominique Langin

Research output: Contribution to journalArticle

454 Citations (Scopus)

Abstract

Adipose tissue produces inflammation and immunity molecules suspected to be involved in obesity-related complications. The pattern of expression and the nutritional regulation of these molecules in humans are poorly understood. We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray and reverse transcription quantitative PCR. The patterns of expression were compared with that of 17 non-obese subjects. We determined whether the regulated genes were expressed in adipocytes or stromavascular fraction cells. Gene expression profiling identified 100 inflammation-related transcripts that are regulated in obese individuals when eating a 28 day VLCD but not a 2 day VLCD. Cluster analysis showed that the pattern of gene expression in obese subjects after 28 day VLCD was closer to the profile of lean subjects than to the pattern of obese subjects before VLCD. Weight loss improves the inflammatory profile of obese subjects through a decrease of pro inflammatory factors and an increase of anti-inflammatory molecules. The genes are expressed mostly in the stromavascular fraction of adipose tissue, which is shown to contain numerous macrophages. The beneficial effect of weight loss on obesity-related complications may be associated with the modification of the inflammatory profile in adipose tissue. - Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J.-D., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello R., Langin, D. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects.

Original languageEnglish
Pages (from-to)1657-1669
Number of pages13
JournalFASEB Journal
Volume18
Issue number14
DOIs
Publication statusPublished - Nov 2004

Fingerprint

very low calorie diet
Caloric Restriction
White Adipose Tissue
white adipose tissue
Weight Loss
weight loss
Genes
inflammation
Tissue
Inflammation
Gene expression
adipose tissue
Adipose Tissue
genes
gene expression
Molecules
obesity
Obesity
reverse transcription
Macrophages

Keywords

  • Calorie restriction
  • Gene profiling
  • Microarray
  • Obesity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., ... Langin, D. (2004). Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects. FASEB Journal, 18(14), 1657-1669. https://doi.org/10.1096/fj.04-2204com

Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects. / Clément, Karine; Viguerie, Nathalie; Poitou, Christine; Carette, Claire; Pelloux, Véronique; Curat, Cyrile A.; Sicard, Audrey; Rome, Sophie; Benis, Arriel; Zucker, Jean Daniel; Vidal, Hubert; Laville, Martine; Barsh, Gregory S.; Basdevant, Arnaud; Stich, Vladimir; Cancello, Raffaella; Langin, Dominique.

In: FASEB Journal, Vol. 18, No. 14, 11.2004, p. 1657-1669.

Research output: Contribution to journalArticle

Clément, K, Viguerie, N, Poitou, C, Carette, C, Pelloux, V, Curat, CA, Sicard, A, Rome, S, Benis, A, Zucker, JD, Vidal, H, Laville, M, Barsh, GS, Basdevant, A, Stich, V, Cancello, R & Langin, D 2004, 'Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects', FASEB Journal, vol. 18, no. 14, pp. 1657-1669. https://doi.org/10.1096/fj.04-2204com
Clément K, Viguerie N, Poitou C, Carette C, Pelloux V, Curat CA et al. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects. FASEB Journal. 2004 Nov;18(14):1657-1669. https://doi.org/10.1096/fj.04-2204com
Clément, Karine ; Viguerie, Nathalie ; Poitou, Christine ; Carette, Claire ; Pelloux, Véronique ; Curat, Cyrile A. ; Sicard, Audrey ; Rome, Sophie ; Benis, Arriel ; Zucker, Jean Daniel ; Vidal, Hubert ; Laville, Martine ; Barsh, Gregory S. ; Basdevant, Arnaud ; Stich, Vladimir ; Cancello, Raffaella ; Langin, Dominique. / Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects. In: FASEB Journal. 2004 ; Vol. 18, No. 14. pp. 1657-1669.
@article{4bd8983112d34426964095a709df9b28,
title = "Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects",
abstract = "Adipose tissue produces inflammation and immunity molecules suspected to be involved in obesity-related complications. The pattern of expression and the nutritional regulation of these molecules in humans are poorly understood. We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray and reverse transcription quantitative PCR. The patterns of expression were compared with that of 17 non-obese subjects. We determined whether the regulated genes were expressed in adipocytes or stromavascular fraction cells. Gene expression profiling identified 100 inflammation-related transcripts that are regulated in obese individuals when eating a 28 day VLCD but not a 2 day VLCD. Cluster analysis showed that the pattern of gene expression in obese subjects after 28 day VLCD was closer to the profile of lean subjects than to the pattern of obese subjects before VLCD. Weight loss improves the inflammatory profile of obese subjects through a decrease of pro inflammatory factors and an increase of anti-inflammatory molecules. The genes are expressed mostly in the stromavascular fraction of adipose tissue, which is shown to contain numerous macrophages. The beneficial effect of weight loss on obesity-related complications may be associated with the modification of the inflammatory profile in adipose tissue. - Cl{\'e}ment, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J.-D., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello R., Langin, D. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects.",
keywords = "Calorie restriction, Gene profiling, Microarray, Obesity",
author = "Karine Cl{\'e}ment and Nathalie Viguerie and Christine Poitou and Claire Carette and V{\'e}ronique Pelloux and Curat, {Cyrile A.} and Audrey Sicard and Sophie Rome and Arriel Benis and Zucker, {Jean Daniel} and Hubert Vidal and Martine Laville and Barsh, {Gregory S.} and Arnaud Basdevant and Vladimir Stich and Raffaella Cancello and Dominique Langin",
year = "2004",
month = "11",
doi = "10.1096/fj.04-2204com",
language = "English",
volume = "18",
pages = "1657--1669",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "14",

}

TY - JOUR

T1 - Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects

AU - Clément, Karine

AU - Viguerie, Nathalie

AU - Poitou, Christine

AU - Carette, Claire

AU - Pelloux, Véronique

AU - Curat, Cyrile A.

AU - Sicard, Audrey

AU - Rome, Sophie

AU - Benis, Arriel

AU - Zucker, Jean Daniel

AU - Vidal, Hubert

AU - Laville, Martine

AU - Barsh, Gregory S.

AU - Basdevant, Arnaud

AU - Stich, Vladimir

AU - Cancello, Raffaella

AU - Langin, Dominique

PY - 2004/11

Y1 - 2004/11

N2 - Adipose tissue produces inflammation and immunity molecules suspected to be involved in obesity-related complications. The pattern of expression and the nutritional regulation of these molecules in humans are poorly understood. We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray and reverse transcription quantitative PCR. The patterns of expression were compared with that of 17 non-obese subjects. We determined whether the regulated genes were expressed in adipocytes or stromavascular fraction cells. Gene expression profiling identified 100 inflammation-related transcripts that are regulated in obese individuals when eating a 28 day VLCD but not a 2 day VLCD. Cluster analysis showed that the pattern of gene expression in obese subjects after 28 day VLCD was closer to the profile of lean subjects than to the pattern of obese subjects before VLCD. Weight loss improves the inflammatory profile of obese subjects through a decrease of pro inflammatory factors and an increase of anti-inflammatory molecules. The genes are expressed mostly in the stromavascular fraction of adipose tissue, which is shown to contain numerous macrophages. The beneficial effect of weight loss on obesity-related complications may be associated with the modification of the inflammatory profile in adipose tissue. - Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J.-D., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello R., Langin, D. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects.

AB - Adipose tissue produces inflammation and immunity molecules suspected to be involved in obesity-related complications. The pattern of expression and the nutritional regulation of these molecules in humans are poorly understood. We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray and reverse transcription quantitative PCR. The patterns of expression were compared with that of 17 non-obese subjects. We determined whether the regulated genes were expressed in adipocytes or stromavascular fraction cells. Gene expression profiling identified 100 inflammation-related transcripts that are regulated in obese individuals when eating a 28 day VLCD but not a 2 day VLCD. Cluster analysis showed that the pattern of gene expression in obese subjects after 28 day VLCD was closer to the profile of lean subjects than to the pattern of obese subjects before VLCD. Weight loss improves the inflammatory profile of obese subjects through a decrease of pro inflammatory factors and an increase of anti-inflammatory molecules. The genes are expressed mostly in the stromavascular fraction of adipose tissue, which is shown to contain numerous macrophages. The beneficial effect of weight loss on obesity-related complications may be associated with the modification of the inflammatory profile in adipose tissue. - Clément, K., Viguerie, N., Poitou, C., Carette, C., Pelloux, V., Curat, C. A., Sicard, A., Rome, S., Benis, A., Zucker, J.-D., Vidal, H., Laville, M., Barsh, G. S., Basdevant, A., Stich, V., Cancello R., Langin, D. Weight loss regulates inflammation-related genes in white adipose tissue of obese subjects.

KW - Calorie restriction

KW - Gene profiling

KW - Microarray

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=7744226149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7744226149&partnerID=8YFLogxK

U2 - 10.1096/fj.04-2204com

DO - 10.1096/fj.04-2204com

M3 - Article

C2 - 15522911

AN - SCOPUS:7744226149

VL - 18

SP - 1657

EP - 1669

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 14

ER -