Wheat germ agglutinin fracture-label of Golgi apparatus membranes in proliferating cells

Lavinia Vittoria Lotti, Antonio Pavan, Patrizia Mancini, Luigi Frati, Maria Rosaria Torrisi

Research output: Contribution to journalArticlepeer-review

Abstract

The thin section fracture-label technique has been recently used to analyze the distribution and compartmentalization of fully glycosylated components on intracellular membranes. Labelling with the lectin wheat germ agglutinin over the freeze-fractured membranes of Golgi apparatus in various secretory and non-secretory cells as well as in human peripheral lymphocytes was always very weak or absent even over the trans-most cisternae. In order to investigate if the labelling density may reflect the cellular activity in membrane biogenesis, we used in this study wheat germ agglutinin fracture-label of rapidly proliferating cells and mitogen-activated lymphocytes. Labelling over the fractured cisternae of the medial and trans portions of the Golgi apparatus was intense. Treatment with cycloheximide of proliferating cells induced a drastic reduction of the labelling over the Golgi cisternae.

Original languageEnglish
Pages (from-to)597-605
Number of pages9
JournalCell Biology International Reports
Volume12
Issue number8
DOIs
Publication statusPublished - 1988

Keywords

  • Fracture-label
  • Freeze-fracture
  • Golgi apparatus

ASJC Scopus subject areas

  • Cell Biology

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