TY - JOUR
T1 - Wheat germ agglutinin fracture-label of Golgi apparatus membranes in proliferating cells
AU - Vittoria Lotti, Lavinia
AU - Pavan, Antonio
AU - Mancini, Patrizia
AU - Frati, Luigi
AU - Rosaria Torrisi, Maria
PY - 1988
Y1 - 1988
N2 - The thin section fracture-label technique has been recently used to analyze the distribution and compartmentalization of fully glycosylated components on intracellular membranes. Labelling with the lectin wheat germ agglutinin over the freeze-fractured membranes of Golgi apparatus in various secretory and non-secretory cells as well as in human peripheral lymphocytes was always very weak or absent even over the trans-most cisternae. In order to investigate if the labelling density may reflect the cellular activity in membrane biogenesis, we used in this study wheat germ agglutinin fracture-label of rapidly proliferating cells and mitogen-activated lymphocytes. Labelling over the fractured cisternae of the medial and trans portions of the Golgi apparatus was intense. Treatment with cycloheximide of proliferating cells induced a drastic reduction of the labelling over the Golgi cisternae.
AB - The thin section fracture-label technique has been recently used to analyze the distribution and compartmentalization of fully glycosylated components on intracellular membranes. Labelling with the lectin wheat germ agglutinin over the freeze-fractured membranes of Golgi apparatus in various secretory and non-secretory cells as well as in human peripheral lymphocytes was always very weak or absent even over the trans-most cisternae. In order to investigate if the labelling density may reflect the cellular activity in membrane biogenesis, we used in this study wheat germ agglutinin fracture-label of rapidly proliferating cells and mitogen-activated lymphocytes. Labelling over the fractured cisternae of the medial and trans portions of the Golgi apparatus was intense. Treatment with cycloheximide of proliferating cells induced a drastic reduction of the labelling over the Golgi cisternae.
KW - Fracture-label
KW - Freeze-fracture
KW - Golgi apparatus
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U2 - 10.1016/0309-1651(88)90152-X
DO - 10.1016/0309-1651(88)90152-X
M3 - Article
C2 - 3197118
AN - SCOPUS:0024064685
VL - 12
SP - 597
EP - 605
JO - Cell Biology International Reports
JF - Cell Biology International Reports
SN - 0309-1651
IS - 8
ER -