Wheat germ agglutinin fracture‐label of lymphocyte plasma and intracellular membranes

Maria Rosaria Torrisi, Antonio Pavan, Lavinia Vittoria Lotti, Luigi Frati

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze‐fracture in fracture‐label techniques: The thin section fracture‐label and the critical point drying fracture‐label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze‐fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze‐fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalJournal of Electron Microscopy Technique
Volume4
Issue number1
DOIs
Publication statusPublished - 1986

Fingerprint

Intracellular Membranes
Wheat Germ Agglutinins
Cell Membrane
Lymphocytes
Histocytochemistry
Lectins
Immunohistochemistry

Keywords

  • Cytochemistry
  • Fracture‐label
  • Freeze‐fracture
  • Wheat germ agglutinin

ASJC Scopus subject areas

  • Anatomy

Cite this

Wheat germ agglutinin fracture‐label of lymphocyte plasma and intracellular membranes. / Torrisi, Maria Rosaria; Pavan, Antonio; Lotti, Lavinia Vittoria; Frati, Luigi.

In: Journal of Electron Microscopy Technique, Vol. 4, No. 1, 1986, p. 41-46.

Research output: Contribution to journalArticle

Torrisi, Maria Rosaria ; Pavan, Antonio ; Lotti, Lavinia Vittoria ; Frati, Luigi. / Wheat germ agglutinin fracture‐label of lymphocyte plasma and intracellular membranes. In: Journal of Electron Microscopy Technique. 1986 ; Vol. 4, No. 1. pp. 41-46.
@article{bd6e64b7f7e14e17a895cb081a7933d3,
title = "Wheat germ agglutinin fracture‐label of lymphocyte plasma and intracellular membranes",
abstract = "Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze‐fracture in fracture‐label techniques: The thin section fracture‐label and the critical point drying fracture‐label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze‐fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze‐fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.",
keywords = "Cytochemistry, Fracture‐label, Freeze‐fracture, Wheat germ agglutinin",
author = "Torrisi, {Maria Rosaria} and Antonio Pavan and Lotti, {Lavinia Vittoria} and Luigi Frati",
year = "1986",
doi = "10.1002/jemt.1060040104",
language = "English",
volume = "4",
pages = "41--46",
journal = "Journal of Electron Microscopy Technique",
issn = "0741-0581",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Wheat germ agglutinin fracture‐label of lymphocyte plasma and intracellular membranes

AU - Torrisi, Maria Rosaria

AU - Pavan, Antonio

AU - Lotti, Lavinia Vittoria

AU - Frati, Luigi

PY - 1986

Y1 - 1986

N2 - Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze‐fracture in fracture‐label techniques: The thin section fracture‐label and the critical point drying fracture‐label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze‐fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze‐fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.

AB - Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze‐fracture in fracture‐label techniques: The thin section fracture‐label and the critical point drying fracture‐label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze‐fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze‐fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.

KW - Cytochemistry

KW - Fracture‐label

KW - Freeze‐fracture

KW - Wheat germ agglutinin

UR - http://www.scopus.com/inward/record.url?scp=84986505784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986505784&partnerID=8YFLogxK

U2 - 10.1002/jemt.1060040104

DO - 10.1002/jemt.1060040104

M3 - Article

AN - SCOPUS:84986505784

VL - 4

SP - 41

EP - 46

JO - Journal of Electron Microscopy Technique

JF - Journal of Electron Microscopy Technique

SN - 0741-0581

IS - 1

ER -