When Does the Lung Die? Time Course of High Energy Phosphate Depletion and Relationship to Lung Viability after "Death"

Andrea M. D'Armini, Eugene J. Tom, Charles S. Roberts, David C. Henke, John J. Lemasters, Thomas M. Egan

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The shortage of lung donors for clinical transplantation could be significantly alleviated if lungs could be retrieved from cadavers hours after death. However, the time course of loss of lung viability after circulatory arrest and organism death remains unclear. To determine postmortem adenine nucleotide tissue levels in the lung and their relationship to lung viability, Sprague-Dawley rats were sacrificed and then ventilated with 100% oxygen (n = 50, O2) or 100% nitrogen (n = 40, N2) or left nonventilated (n = 50). Lungs from control rats (n = 20) were retrieved immediately after sacrifice. Lungs in the three study groups were retrieved at successive intervals postmortem. Adenine nucleotides (ATP, ADP, and AMP) and hypoxanthine and xanthine metabolites of adenosine were extracted from lung tissue and measured using high-performance liquid chromatography. Pulmonary parenchymal cell viability was quantified by pulmonary artery infusion of trypan blue vital dye in the contralateral lung of each animal. By 4 hr postmortem, viability was 85 ± 1% in the O2-ventilated cadaver rat lungs, significantly higher than in the N2-ventilated (43 ± 8%) and in the nonventilated (48 ± 4%) lungs, where the percentage of viable cells was similar. All of the groups showed a time-dependent decrement in ATP levels and total adenine nucleotide (TAN) levels after death, but this was markedly attenuated in O2-ventilated cadaveric rat long. In the control group, the level of TAN was 10.2 ± 0.9 μmoles/g dry weight, while TAN levels fell to 5.8 ± 0.9 in O2-ventilated lungs, 4.4 ± 0.6 in N2-ventilated lungs, and 3.3 ± 0.2 in nonventilated lungs 4 hr after death. As TAN levels declined, lung tissue levels of hypoxanthine and xanthine increased after death, more so in nonventilated cadaver longs, and less so in O2-ventilated cadaveric rat lung. TAN levels correlated well with the viability of pulmonary parenchymal cells in the postmortem lung across all of the groups and times postmortem (r = 0.88). O2 ventilation of the nonperfused lung preserved adenine nucleotides, compared with N2 ventilation or nonventilation, and this correlated with increased pulmonary parenchymal cell viability. Viability of the postmortem lung may allow retrieval from cadavers and successful transplantation in the clinical setting.

Original languageEnglish
Pages (from-to)468-474
Number of pages7
JournalJournal of Surgical Research
Volume59
Issue number4
DOIs
Publication statusPublished - Oct 1995

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Phosphates
Lung
Adenine Nucleotides
Cadaver
Hypoxanthine
Xanthine
Ventilation
Cell Survival
Adenosine Triphosphate
Transplantation
Trypan Blue
Adenosine Monophosphate
Adenosine
Adenosine Diphosphate
Pulmonary Artery
Sprague Dawley Rats

ASJC Scopus subject areas

  • Surgery

Cite this

When Does the Lung Die? Time Course of High Energy Phosphate Depletion and Relationship to Lung Viability after "Death". / D'Armini, Andrea M.; Tom, Eugene J.; Roberts, Charles S.; Henke, David C.; Lemasters, John J.; Egan, Thomas M.

In: Journal of Surgical Research, Vol. 59, No. 4, 10.1995, p. 468-474.

Research output: Contribution to journalArticle

D'Armini, Andrea M. ; Tom, Eugene J. ; Roberts, Charles S. ; Henke, David C. ; Lemasters, John J. ; Egan, Thomas M. / When Does the Lung Die? Time Course of High Energy Phosphate Depletion and Relationship to Lung Viability after "Death". In: Journal of Surgical Research. 1995 ; Vol. 59, No. 4. pp. 468-474.
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