TY - JOUR
T1 - Whole blood endotoxin responsiveness in patients with chronic heart failure
T2 - The importance of serum lipoproteins
AU - Sharma, Rakesh
AU - Von Haehling, Stephan
AU - Rauchhaus, Mathias
AU - Bolger, Aidan P.
AU - Genth-Zotz, Sabine
AU - Doehner, Wolfram
AU - Oliver, Brian
AU - Poole-Wilson, Philip A.
AU - Volk, Hans Dieter
AU - Coats, Andrew J S
AU - Adcock, Ian M.
AU - Anker, Stefan D.
PY - 2005/6
Y1 - 2005/6
N2 - Background: Endotoxin [lipopolysaccharide (LPS)] may be an important stimulus for cytokine release in patients with chronic heart failure (CHF). We sought to investigate the relationship between whole blood endotoxin responsiveness and serum lipoprotein concentrations. It is not known if low-dose LPS is sufficient to stimulate immune activation. Methods and results: Whole blood from 32 CHF patients (mean age 66±2 years, NYHA class 2.7±0.2, five female) and 11 healthy control subjects (mean age 47±4 years, six female) was stimulated with LPS at nine different concentrations (0.001 to 10 ng/mL), and tumor necrosis factor (TNF-α) release was quantified. Reference standard endotoxin at concentrations of 0, 0.6, 1, and 3 EU/ml was added to whole blood from nine CHF patients (age 64±9.1 years, all NYHA class II, eight male) and incubated for 6 h, the TNF-α production being measured. Serum lipoproteins were quantified using standard techniques. In CHF patients, there was an inverse relationship between whole blood TNF-α release and serum cholesterol which was strongest at 0.6 ng/mL of LPS (r=-0.53, p=0.002). A similar although weaker relationship was found for serum HDL. No such correlation was found in healthy subjects or with serum LDL (all r2
AB - Background: Endotoxin [lipopolysaccharide (LPS)] may be an important stimulus for cytokine release in patients with chronic heart failure (CHF). We sought to investigate the relationship between whole blood endotoxin responsiveness and serum lipoprotein concentrations. It is not known if low-dose LPS is sufficient to stimulate immune activation. Methods and results: Whole blood from 32 CHF patients (mean age 66±2 years, NYHA class 2.7±0.2, five female) and 11 healthy control subjects (mean age 47±4 years, six female) was stimulated with LPS at nine different concentrations (0.001 to 10 ng/mL), and tumor necrosis factor (TNF-α) release was quantified. Reference standard endotoxin at concentrations of 0, 0.6, 1, and 3 EU/ml was added to whole blood from nine CHF patients (age 64±9.1 years, all NYHA class II, eight male) and incubated for 6 h, the TNF-α production being measured. Serum lipoproteins were quantified using standard techniques. In CHF patients, there was an inverse relationship between whole blood TNF-α release and serum cholesterol which was strongest at 0.6 ng/mL of LPS (r=-0.53, p=0.002). A similar although weaker relationship was found for serum HDL. No such correlation was found in healthy subjects or with serum LDL (all r2
KW - Heart failure
KW - Immune system
KW - Lipids
KW - Lipopolysaccharide
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U2 - 10.1016/j.ejheart.2004.09.013
DO - 10.1016/j.ejheart.2004.09.013
M3 - Article
C2 - 15921783
AN - SCOPUS:19544394823
VL - 7
SP - 479
EP - 484
JO - European Journal of Heart Failure
JF - European Journal of Heart Failure
SN - 1388-9842
IS - 4
ER -