Whole blood endotoxin responsiveness in patients with chronic heart failure: The importance of serum lipoproteins

Rakesh Sharma, Stephan Von Haehling, Mathias Rauchhaus, Aidan P. Bolger, Sabine Genth-Zotz, Wolfram Doehner, Brian Oliver, Philip A. Poole-Wilson, Hans Dieter Volk, Andrew J S Coats, Ian M. Adcock, Stefan D. Anker

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Endotoxin [lipopolysaccharide (LPS)] may be an important stimulus for cytokine release in patients with chronic heart failure (CHF). We sought to investigate the relationship between whole blood endotoxin responsiveness and serum lipoprotein concentrations. It is not known if low-dose LPS is sufficient to stimulate immune activation. Methods and results: Whole blood from 32 CHF patients (mean age 66±2 years, NYHA class 2.7±0.2, five female) and 11 healthy control subjects (mean age 47±4 years, six female) was stimulated with LPS at nine different concentrations (0.001 to 10 ng/mL), and tumor necrosis factor (TNF-α) release was quantified. Reference standard endotoxin at concentrations of 0, 0.6, 1, and 3 EU/ml was added to whole blood from nine CHF patients (age 64±9.1 years, all NYHA class II, eight male) and incubated for 6 h, the TNF-α production being measured. Serum lipoproteins were quantified using standard techniques. In CHF patients, there was an inverse relationship between whole blood TNF-α release and serum cholesterol which was strongest at 0.6 ng/mL of LPS (r=-0.53, p=0.002). A similar although weaker relationship was found for serum HDL. No such correlation was found in healthy subjects or with serum LDL (all r2

Original languageEnglish
Pages (from-to)479-484
Number of pages6
JournalEuropean Journal of Heart Failure
Volume7
Issue number4
DOIs
Publication statusPublished - Jun 2005

Keywords

  • Heart failure
  • Immune system
  • Lipids
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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