Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease

Patrizia Risé, Sonia Volpi, Claudio Colombo, Rita Francesca Padoan, Ciro D'Orazio, Silvia Ghezzi, Paola Melotti, Veronica Bennato, Carlo Agostoni, Baroukh Maurice Assael, Claudio Galli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: To assess fatty acid (FA) profiles in whole blood of 90 cystic fibrosis patients (CF) and 30 control subjects (C) and to correlate FA changes to the severity of respiratory disease. Methods: Whole blood FA were assessed by GC with a micromethod-based analysis. Results: Saturated and monounsaturated FA are higher, whereas polyunsaturated FA are lower in CF versus C with reduction of total n-6 FA, 22:5n-3 and 22:6n-3 (DHA). The product of linoleic acid (LA) × DHA, proposed as a marker for the disease, is 30% lower in CF than in C. Correlations with the severity of the respiratory disease are present for different FA and for the LA × DHA product. There is a reduction of Δ5 desaturase activity in CF, greater in severe disease, suggesting a basic metabolic alteration. Conclusions: The micromethod-based analysis of blood FA facilitates the assessment of the FA status while confirming alterations of FA profiles already reported in specific blood compartments of CF.

Original languageEnglish
Pages (from-to)228-233
Number of pages6
JournalJournal of Cystic Fibrosis
Volume9
Issue number3
DOIs
Publication statusPublished - May 2010

Fingerprint

Cystic Fibrosis
Lung Diseases
Fatty Acids
Linoleic Acid
Monounsaturated Fatty Acids
Unsaturated Fatty Acids

Keywords

  • Cystic fibrosis
  • Fatty acid desaturation
  • Pulmonary disease
  • Whole blood fatty acids

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pediatrics, Perinatology, and Child Health
  • Medicine(all)

Cite this

Risé, P., Volpi, S., Colombo, C., Padoan, R. F., D'Orazio, C., Ghezzi, S., ... Galli, C. (2010). Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease. Journal of Cystic Fibrosis, 9(3), 228-233. https://doi.org/10.1016/j.jcf.2010.03.002

Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease. / Risé, Patrizia; Volpi, Sonia; Colombo, Claudio; Padoan, Rita Francesca; D'Orazio, Ciro; Ghezzi, Silvia; Melotti, Paola; Bennato, Veronica; Agostoni, Carlo; Assael, Baroukh Maurice; Galli, Claudio.

In: Journal of Cystic Fibrosis, Vol. 9, No. 3, 05.2010, p. 228-233.

Research output: Contribution to journalArticle

Risé, P, Volpi, S, Colombo, C, Padoan, RF, D'Orazio, C, Ghezzi, S, Melotti, P, Bennato, V, Agostoni, C, Assael, BM & Galli, C 2010, 'Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease', Journal of Cystic Fibrosis, vol. 9, no. 3, pp. 228-233. https://doi.org/10.1016/j.jcf.2010.03.002
Risé, Patrizia ; Volpi, Sonia ; Colombo, Claudio ; Padoan, Rita Francesca ; D'Orazio, Ciro ; Ghezzi, Silvia ; Melotti, Paola ; Bennato, Veronica ; Agostoni, Carlo ; Assael, Baroukh Maurice ; Galli, Claudio. / Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease. In: Journal of Cystic Fibrosis. 2010 ; Vol. 9, No. 3. pp. 228-233.
@article{01dfe874127940d3ba7dabfc82e3b85c,
title = "Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease",
abstract = "Objectives: To assess fatty acid (FA) profiles in whole blood of 90 cystic fibrosis patients (CF) and 30 control subjects (C) and to correlate FA changes to the severity of respiratory disease. Methods: Whole blood FA were assessed by GC with a micromethod-based analysis. Results: Saturated and monounsaturated FA are higher, whereas polyunsaturated FA are lower in CF versus C with reduction of total n-6 FA, 22:5n-3 and 22:6n-3 (DHA). The product of linoleic acid (LA) × DHA, proposed as a marker for the disease, is 30{\%} lower in CF than in C. Correlations with the severity of the respiratory disease are present for different FA and for the LA × DHA product. There is a reduction of Δ5 desaturase activity in CF, greater in severe disease, suggesting a basic metabolic alteration. Conclusions: The micromethod-based analysis of blood FA facilitates the assessment of the FA status while confirming alterations of FA profiles already reported in specific blood compartments of CF.",
keywords = "Cystic fibrosis, Fatty acid desaturation, Pulmonary disease, Whole blood fatty acids",
author = "Patrizia Ris{\'e} and Sonia Volpi and Claudio Colombo and Padoan, {Rita Francesca} and Ciro D'Orazio and Silvia Ghezzi and Paola Melotti and Veronica Bennato and Carlo Agostoni and Assael, {Baroukh Maurice} and Claudio Galli",
year = "2010",
month = "5",
doi = "10.1016/j.jcf.2010.03.002",
language = "English",
volume = "9",
pages = "228--233",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Whole blood fatty acid analysis with micromethod in cystic fibrosis and pulmonary disease

AU - Risé, Patrizia

AU - Volpi, Sonia

AU - Colombo, Claudio

AU - Padoan, Rita Francesca

AU - D'Orazio, Ciro

AU - Ghezzi, Silvia

AU - Melotti, Paola

AU - Bennato, Veronica

AU - Agostoni, Carlo

AU - Assael, Baroukh Maurice

AU - Galli, Claudio

PY - 2010/5

Y1 - 2010/5

N2 - Objectives: To assess fatty acid (FA) profiles in whole blood of 90 cystic fibrosis patients (CF) and 30 control subjects (C) and to correlate FA changes to the severity of respiratory disease. Methods: Whole blood FA were assessed by GC with a micromethod-based analysis. Results: Saturated and monounsaturated FA are higher, whereas polyunsaturated FA are lower in CF versus C with reduction of total n-6 FA, 22:5n-3 and 22:6n-3 (DHA). The product of linoleic acid (LA) × DHA, proposed as a marker for the disease, is 30% lower in CF than in C. Correlations with the severity of the respiratory disease are present for different FA and for the LA × DHA product. There is a reduction of Δ5 desaturase activity in CF, greater in severe disease, suggesting a basic metabolic alteration. Conclusions: The micromethod-based analysis of blood FA facilitates the assessment of the FA status while confirming alterations of FA profiles already reported in specific blood compartments of CF.

AB - Objectives: To assess fatty acid (FA) profiles in whole blood of 90 cystic fibrosis patients (CF) and 30 control subjects (C) and to correlate FA changes to the severity of respiratory disease. Methods: Whole blood FA were assessed by GC with a micromethod-based analysis. Results: Saturated and monounsaturated FA are higher, whereas polyunsaturated FA are lower in CF versus C with reduction of total n-6 FA, 22:5n-3 and 22:6n-3 (DHA). The product of linoleic acid (LA) × DHA, proposed as a marker for the disease, is 30% lower in CF than in C. Correlations with the severity of the respiratory disease are present for different FA and for the LA × DHA product. There is a reduction of Δ5 desaturase activity in CF, greater in severe disease, suggesting a basic metabolic alteration. Conclusions: The micromethod-based analysis of blood FA facilitates the assessment of the FA status while confirming alterations of FA profiles already reported in specific blood compartments of CF.

KW - Cystic fibrosis

KW - Fatty acid desaturation

KW - Pulmonary disease

KW - Whole blood fatty acids

UR - http://www.scopus.com/inward/record.url?scp=77953356014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953356014&partnerID=8YFLogxK

U2 - 10.1016/j.jcf.2010.03.002

DO - 10.1016/j.jcf.2010.03.002

M3 - Article

C2 - 20338832

AN - SCOPUS:77953356014

VL - 9

SP - 228

EP - 233

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - 3

ER -