Abstract
The analytical performance of the Stratus Folate assay for intra-erythrocyte folate determination in normal subjects and in patients affected by folate-related diseases was compared with that of the radioassay (DPC) routinely employed by us. Folate concentrations were measured in freshly obtained EDTA whole blood from 100 subjects. Haemolysis was performed with the appropriate lysis reagent. In addition, to compare two different haemolysis procedures folate determination was carried out in 51 samples haemolysed according to the two procedures in parallel. Data were analyzed using Wilcoxon's test and standardized principal component analysis. Stratus Folate assay and radioassay performances were comparable in terms of analytical characteristics as well as in individual intraerythrocyte folate values across the range of whole blood concentrations examined in the survey. Significant differences were detected between the two different haemolysis procedures only for the radioassay. In conclusion, we observed no significant differences between the two folate determination methods despite their different analytical principles, which indicates the suitability of routine use of the automated non-isotopic Stratus Folate assay for clinical purposes. Moreover, with the latter assay the laboratory staff could choose the more convenient haemolysis procedure.
| Original language | English |
|---|---|
| Pages (from-to) | 781-785 |
| Number of pages | 5 |
| Journal | European Journal of Clinical Chemistry and Clinical Biochemistry |
| Volume | 35 |
| Issue number | 10 |
| Publication status | Published - 1997 |
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ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Whole blood folate concentrations : Comparison between Stratus Folate (DADE) and radioassay (DPC) methods. / Bamonti-Catena, F.; Porcella, A.; Pomati, M.; Franzini, C.; Rosina, M.; Cavalca, V.; Maiolo, A. T.
In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 35, No. 10, 1997, p. 781-785.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Whole blood folate concentrations
T2 - Comparison between Stratus Folate (DADE) and radioassay (DPC) methods
AU - Bamonti-Catena, F.
AU - Porcella, A.
AU - Pomati, M.
AU - Franzini, C.
AU - Rosina, M.
AU - Cavalca, V.
AU - Maiolo, A. T.
PY - 1997
Y1 - 1997
N2 - The analytical performance of the Stratus Folate assay for intra-erythrocyte folate determination in normal subjects and in patients affected by folate-related diseases was compared with that of the radioassay (DPC) routinely employed by us. Folate concentrations were measured in freshly obtained EDTA whole blood from 100 subjects. Haemolysis was performed with the appropriate lysis reagent. In addition, to compare two different haemolysis procedures folate determination was carried out in 51 samples haemolysed according to the two procedures in parallel. Data were analyzed using Wilcoxon's test and standardized principal component analysis. Stratus Folate assay and radioassay performances were comparable in terms of analytical characteristics as well as in individual intraerythrocyte folate values across the range of whole blood concentrations examined in the survey. Significant differences were detected between the two different haemolysis procedures only for the radioassay. In conclusion, we observed no significant differences between the two folate determination methods despite their different analytical principles, which indicates the suitability of routine use of the automated non-isotopic Stratus Folate assay for clinical purposes. Moreover, with the latter assay the laboratory staff could choose the more convenient haemolysis procedure.
AB - The analytical performance of the Stratus Folate assay for intra-erythrocyte folate determination in normal subjects and in patients affected by folate-related diseases was compared with that of the radioassay (DPC) routinely employed by us. Folate concentrations were measured in freshly obtained EDTA whole blood from 100 subjects. Haemolysis was performed with the appropriate lysis reagent. In addition, to compare two different haemolysis procedures folate determination was carried out in 51 samples haemolysed according to the two procedures in parallel. Data were analyzed using Wilcoxon's test and standardized principal component analysis. Stratus Folate assay and radioassay performances were comparable in terms of analytical characteristics as well as in individual intraerythrocyte folate values across the range of whole blood concentrations examined in the survey. Significant differences were detected between the two different haemolysis procedures only for the radioassay. In conclusion, we observed no significant differences between the two folate determination methods despite their different analytical principles, which indicates the suitability of routine use of the automated non-isotopic Stratus Folate assay for clinical purposes. Moreover, with the latter assay the laboratory staff could choose the more convenient haemolysis procedure.
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M3 - Article
C2 - 9368797
AN - SCOPUS:0030669744
VL - 35
SP - 781
EP - 785
JO - European Journal of Clinical Chemistry and Clinical Biochemistry
JF - European Journal of Clinical Chemistry and Clinical Biochemistry
SN - 0939-4974
IS - 10
ER -