Whole-cell patch-clamp recording of voltage-sensitive Ca2+ channel currents in single cells: heterologous expression systems and neurones.

Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, Andrew D. Randall

Research output: Contribution to journalArticle

Abstract

Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of many cell types, including neurones, skeletal, cardiac and smooth muscle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and biophysics of VSCC proteins with a level of precision rarely surpassed in cell biological studies. With best practice voltage-dependent gating behaviors of VSCCs can be interrogated with temporal resolution

Original languageEnglish
Pages (from-to)123-148
Number of pages26
JournalMethods in molecular biology (Clifton, N.J.)
Volume937
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Medicine(all)

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