Whole exome sequencing of rare aggressive breast cancer histologies

Maria Vittoria Dieci, Veronika Smutná, Véronique Scott, Guangliang Yin, Ran Xu, Philippe Vielh, Marie Christine Mathieu, Cécile Vicier, Melanie Laporte, Francoise Drusch, Valentina Guarneri, Pierfranco Conte, Suzette Delaloge, Ludovic Lacroix, Olivia Fromigué, Fabrice André, Celine Lefebvre

Research output: Contribution to journalArticle

Abstract

Little is known about mutational landscape of rare breast cancer (BC) subtypes. The aim of the study was to apply next generation sequencing to three different subtypes of rare BCs in order to identify new genes related to cancer progression. We performed whole exome and targeted sequencing of 29 micropapillary, 23 metaplastic, and 27 pleomorphic lobular BCs. Micropapillary BCs exhibit a profile comparable to common BCs: PIK3CA, TP53, GATA3, and MAP2K4 were the most frequently mutated genes. Metaplastic BCs presented a high frequency of TP53 (78 %) and PIK3CA (48 %) mutations and were recurrently mutated on KDM6A (13 %), a gene involved in histone demethylation. Pleomorphic lobular carcinoma exhibited high mutation rate of PIK3CA (30 %), TP53 (22 %), and CDH1 (41 %) and also presented mutations in PYGM, a gene involved in glycogen metabolism, in 8 out of 27 samples (30 %). Further analyses of publicly available datasets showed that PYGM is dramatically underexpressed in common cancers as compared to normal tissues and that low expression in tumors is correlated with poor relapse-free survival. Immunohistochemical staining on formalin-fixed paraffin-embedded tissues available in our cohort of patients confirmed higher PYGM expression in normal breast tissue compared to equivalent tumoral zone. Next generation sequencing methods applied on rare cancer subtypes can serve as a useful tool in order to uncover new potential therapeutic targets. Sequencing of pleomorphic lobular carcinoma identified a high rate of alterations in PYGM. These findings emphasize the role of glycogen metabolism in cancer progression.

Original languageEnglish
Pages (from-to)21-32
Number of pages12
JournalBreast Cancer Research and Treatment
Volume156
Issue number1
DOIs
Publication statusPublished - Feb 1 2016

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Keywords

  • Glycogen metabolism
  • Metaplastic breast cancer
  • Micropapillary breast cancer
  • Pleomorphic lobular breast cancer
  • PYGM
  • Sequencing

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Dieci, M. V., Smutná, V., Scott, V., Yin, G., Xu, R., Vielh, P., Mathieu, M. C., Vicier, C., Laporte, M., Drusch, F., Guarneri, V., Conte, P., Delaloge, S., Lacroix, L., Fromigué, O., André, F., & Lefebvre, C. (2016). Whole exome sequencing of rare aggressive breast cancer histologies. Breast Cancer Research and Treatment, 156(1), 21-32. https://doi.org/10.1007/s10549-016-3718-y