Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana

Anuradha Chowdhary, Ferry Hagen, Cheshta Sharma, Abdullah M S Al-Hatmi, Letterio Giuffrè, Domenico Giosa, Shangrong Fan, Hamid Badali, Maria Rosa Felice, Sybren de Hoog, Jacques F Meis, Orazio Romeo

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Abstract

This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating-type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this amino sugar in this yeast. Multi-locus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb, SYA1 and VPS13 loci which contributed significantly to the classification of our set of C. africana strains into six existing diploid sequence types. Amplified fragment length polymorphism fingerprint analysis yielded greater genotypic heterogeneity among the C. africana strains. Overall the data reported here show that in C. africana genetic diversity occurs and the existence of this intriguing group of C. albicans strains with specific phenotypes associated could be useful for future comparative studies in order to better understand the genetics and evolution of this important human pathogen.

Original languageEnglish
Article number556
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 2017

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Amplified Fragment Length Polymorphism Analysis
Candida albicans
Candida
Genome
Amino Sugars
Acetylglucosamine
Molecular Evolution
Essential Genes
Dermatoglyphics
Diploidy
Sequence Analysis
Yeasts
Genotype
Phenotype
Genes

Keywords

  • Journal Article

Cite this

Chowdhary, A., Hagen, F., Sharma, C., Al-Hatmi, A. M. S., Giuffrè, L., Giosa, D., ... Romeo, O. (2017). Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana. Frontiers in Microbiology, 8, [556]. https://doi.org/10.3389/fmicb.2017.00556

Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana. / Chowdhary, Anuradha; Hagen, Ferry; Sharma, Cheshta; Al-Hatmi, Abdullah M S; Giuffrè, Letterio; Giosa, Domenico; Fan, Shangrong; Badali, Hamid; Felice, Maria Rosa; de Hoog, Sybren; Meis, Jacques F; Romeo, Orazio.

In: Frontiers in Microbiology, Vol. 8, 556, 2017.

Research output: Contribution to journalArticle

Chowdhary, A, Hagen, F, Sharma, C, Al-Hatmi, AMS, Giuffrè, L, Giosa, D, Fan, S, Badali, H, Felice, MR, de Hoog, S, Meis, JF & Romeo, O 2017, 'Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana', Frontiers in Microbiology, vol. 8, 556. https://doi.org/10.3389/fmicb.2017.00556
Chowdhary, Anuradha ; Hagen, Ferry ; Sharma, Cheshta ; Al-Hatmi, Abdullah M S ; Giuffrè, Letterio ; Giosa, Domenico ; Fan, Shangrong ; Badali, Hamid ; Felice, Maria Rosa ; de Hoog, Sybren ; Meis, Jacques F ; Romeo, Orazio. / Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana. In: Frontiers in Microbiology. 2017 ; Vol. 8.
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