Abstract
Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.
| Original language | English |
|---|---|
| Pages (from-to) | 375-81 |
| Number of pages | 7 |
| Journal | Journal of Hospital Infection |
| Volume | 93 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 2016 |
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Keywords
- Cross Infection
- Disease Outbreaks
- Disease Transmission, Infectious
- Genome, Bacterial
- Hospitals, Pediatric
- Humans
- Infant
- Infant, Newborn
- Methicillin-Resistant Staphylococcus aureus
- Molecular Epidemiology
- Molecular Typing
- Sequence Analysis, DNA
- Staphylococcal Infections
- Evaluation Studies
- Journal Article
Cite this
Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting. / Ugolotti, E; Larghero, P.; Vanni, I; Bandettini, R; Tripodi, G; Melioli, G.; Di Marco, E; Raso, A; Biassoni, R.
In: Journal of Hospital Infection, Vol. 93, No. 4, 08.2016, p. 375-81.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting
AU - Ugolotti, E
AU - Larghero, P.
AU - Vanni, I
AU - Bandettini, R
AU - Tripodi, G
AU - Melioli, G.
AU - Di Marco, E
AU - Raso, A
AU - Biassoni, R
N1 - Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.
PY - 2016/8
Y1 - 2016/8
N2 - Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.
AB - Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.
KW - Cross Infection
KW - Disease Outbreaks
KW - Disease Transmission, Infectious
KW - Genome, Bacterial
KW - Hospitals, Pediatric
KW - Humans
KW - Infant
KW - Infant, Newborn
KW - Methicillin-Resistant Staphylococcus aureus
KW - Molecular Epidemiology
KW - Molecular Typing
KW - Sequence Analysis, DNA
KW - Staphylococcal Infections
KW - Evaluation Studies
KW - Journal Article
U2 - 10.1016/j.jhin.2016.04.003
DO - 10.1016/j.jhin.2016.04.003
M3 - Article
VL - 93
SP - 375
EP - 381
JO - Journal of Hospital Infection
JF - Journal of Hospital Infection
SN - 0195-6701
IS - 4
ER -