Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting

E Ugolotti; P. Larghero; I Vanni; R Bandettini; G Tripodi; G. Melioli; E Di Marco; A Raso; R Biassoni

doi:10.1016/j.jhin.2016.04.003

Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting

E Ugolotti, P. Larghero, I Vanni, R Bandettini, G Tripodi, G. Melioli, E Di Marco, A Raso, R Biassoni

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.

Original language	English
Pages (from-to)	375-81
Number of pages	7
Journal	Journal of Hospital Infection
Volume	93
Issue number	4
DOIs	https://doi.org/10.1016/j.jhin.2016.04.003
Publication status	Published - Aug 2016

Keywords

Cross Infection
Disease Outbreaks
Disease Transmission, Infectious
Genome, Bacterial
Hospitals, Pediatric
Humans
Infant
Infant, Newborn
Methicillin-Resistant Staphylococcus aureus
Molecular Epidemiology
Molecular Typing
Sequence Analysis, DNA
Staphylococcal Infections
Evaluation Studies
Journal Article

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Ugolotti, E., Larghero, P., Vanni, I., Bandettini, R., Tripodi, G., Melioli, G., Di Marco, E., Raso, A., & Biassoni, R. (2016). Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting. Journal of Hospital Infection, 93(4), 375-81. https://doi.org/10.1016/j.jhin.2016.04.003

Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting. / Ugolotti, E; Larghero, P.; Vanni, I; Bandettini, R ; Tripodi, G; Melioli, G.; Di Marco, E; Raso, A; Biassoni, R.

In: Journal of Hospital Infection, Vol. 93, No. 4, 08.2016, p. 375-81.

Research output: Contribution to journal › Article › peer-review

Ugolotti, E, Larghero, P, Vanni, I, Bandettini, R , Tripodi, G, Melioli, G, Di Marco, E, Raso, A & Biassoni, R 2016, 'Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting', Journal of Hospital Infection, vol. 93, no. 4, pp. 375-81. https://doi.org/10.1016/j.jhin.2016.04.003

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abstract = "Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.",

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KW - Disease Outbreaks

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KW - Genome, Bacterial

KW - Hospitals, Pediatric

KW - Humans

KW - Infant

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KW - Methicillin-Resistant Staphylococcus aureus

KW - Molecular Epidemiology

KW - Molecular Typing

KW - Sequence Analysis, DNA

KW - Staphylococcal Infections

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