Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting

E Ugolotti, P. Larghero, I Vanni, R Bandettini, G Tripodi, G. Melioli, E Di Marco, A Raso, R Biassoni

Research output: Contribution to journalArticle

Abstract

Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.

Original languageEnglish
Pages (from-to)375-81
Number of pages7
JournalJournal of Hospital Infection
Volume93
Issue number4
DOIs
Publication statusPublished - Aug 2016

Fingerprint

Methicillin
Disease Outbreaks
Staphylococcus aureus
Genome
Pediatrics
Pulsed Field Gel Electrophoresis
Cross Infection

Keywords

  • Cross Infection
  • Disease Outbreaks
  • Disease Transmission, Infectious
  • Genome, Bacterial
  • Hospitals, Pediatric
  • Humans
  • Infant
  • Infant, Newborn
  • Methicillin-Resistant Staphylococcus aureus
  • Molecular Epidemiology
  • Molecular Typing
  • Sequence Analysis, DNA
  • Staphylococcal Infections
  • Evaluation Studies
  • Journal Article

Cite this

@article{d8d32b717eee42ffb4e768b7b59e0fdb,
title = "Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting",
abstract = "Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.",
keywords = "Cross Infection, Disease Outbreaks, Disease Transmission, Infectious, Genome, Bacterial, Hospitals, Pediatric, Humans, Infant, Infant, Newborn, Methicillin-Resistant Staphylococcus aureus, Molecular Epidemiology, Molecular Typing, Sequence Analysis, DNA, Staphylococcal Infections, Evaluation Studies, Journal Article",
author = "E Ugolotti and P. Larghero and I Vanni and R Bandettini and G Tripodi and G. Melioli and {Di Marco}, E and A Raso and R Biassoni",
note = "Copyright {\circledC} 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.",
year = "2016",
month = "8",
doi = "10.1016/j.jhin.2016.04.003",
language = "English",
volume = "93",
pages = "375--81",
journal = "Journal of Hospital Infection",
issn = "0195-6701",
publisher = "W.B. Saunders Ltd",
number = "4",

}

TY - JOUR

T1 - Whole-genome sequencing as standard practice for the analysis of clonality in outbreaks of meticillin-resistant Staphylococcus aureus in a paediatric setting

AU - Ugolotti, E

AU - Larghero, P.

AU - Vanni, I

AU - Bandettini, R

AU - Tripodi, G

AU - Melioli, G.

AU - Di Marco, E

AU - Raso, A

AU - Biassoni, R

N1 - Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

PY - 2016/8

Y1 - 2016/8

N2 - Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.

AB - Meticillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-associated infections. This study investigated the potential use of whole-genome sequencing (WGS) for surveillance purposes by re-examining MRSA strains related to past outbreaks among hospitalized paediatric patients. WGS data ameliorated the genotypic profile previously obtained with Sanger sequencing and pulsed-field gel electrophoresis typing, and discriminated between strains that were related and unrelated to the outbreaks. This allowed strain clonality to be defined with a higher level of resolution than achieved previously. This study demonstrates the potential of WGS to trace hospital outbreaks, which may lead to WGS becoming standard practice in outbreak investigations.

KW - Cross Infection

KW - Disease Outbreaks

KW - Disease Transmission, Infectious

KW - Genome, Bacterial

KW - Hospitals, Pediatric

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Methicillin-Resistant Staphylococcus aureus

KW - Molecular Epidemiology

KW - Molecular Typing

KW - Sequence Analysis, DNA

KW - Staphylococcal Infections

KW - Evaluation Studies

KW - Journal Article

U2 - 10.1016/j.jhin.2016.04.003

DO - 10.1016/j.jhin.2016.04.003

M3 - Article

VL - 93

SP - 375

EP - 381

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

IS - 4

ER -