Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes

Samuel Aparicio, Jarrod Chapman, Elia Stupka, Nik Putnam, Jer ming Chia, Paramvir Dehal, Alan Christoffels, Sam Rash, Shawn Hoon, Arian Smit, Maarten D. Sollewijn Gelpke, Jared Roach, Tania Oh, Isaac Y. Ho, Marie Wong, Chris Detter, Frans Verhoef, Paul Predki, Alice Tay, Susan LucasPaul Richardson, Sarah F. Smith, Melody S. Clark, Yvonne J K Edwards, Norman Doggett, Andrey Zharkikh, Sean V. Tavtigian, Dmitry Pruss, Mary Barnstead, Cheryl Evans, Holly Baden, Justin Powell, Gustavo Glusman, Lee Rowen, Leroy Hood, Y. H. Tan, Greg Elgar, Trevor Hawkins, Byrappa Venkatesh, Daniel Rokhsar, Sydney Brenner

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Abstract

The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some "giant" genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.

Original languageEnglish
Pages (from-to)1301-1310
Number of pages10
JournalScience
Volume297
Issue number5585
DOIs
Publication statusPublished - Aug 23 2002

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ASJC Scopus subject areas

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Cite this

Aparicio, S., Chapman, J., Stupka, E., Putnam, N., Chia, J. M., Dehal, P., Christoffels, A., Rash, S., Hoon, S., Smit, A., Sollewijn Gelpke, M. D., Roach, J., Oh, T., Ho, I. Y., Wong, M., Detter, C., Verhoef, F., Predki, P., Tay, A., ... Brenner, S. (2002). Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science, 297(5585), 1301-1310. https://doi.org/10.1126/science.1072104